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PCR amplification and phylogenetic analysis of groESL operon sequences from Ehrlichia ewingii and Ehrlichia muris.

ABSTRACT: Broad-range PCR primers were used to amplify part of the groESL operon of the canine pathogen Ehrlichia ewingii, recently recognized as a human pathogen, and the murine pathogen Ehrlichia muris. Phylogenetic analysis supported the relationships among Ehrlichia species previously determined by comparison of 16S rRNA gene sequences. These sequences provide additional PCR targets for species for which few gene sequences have been determined.

SUBMITTER: Sumner JW

PROVIDER: S-EPMC87017 | biostudies-literature | 2000 Jul

REPOSITORIES: biostudies-literature

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PCR amplification and phylogenetic analysis of groESL operon sequences from Ehrlichia ewingii and Ehrlichia muris.

Sumner J W JW Storch G A GA Buller R S RS Liddell A M AM Stockham S L SL Rikihisa Y Y Messenger S S Paddock C D CD

Journal of clinical microbiology 20000701 7

Broad-range PCR primers were used to amplify part of the groESL operon of the canine pathogen Ehrlichia ewingii, recently recognized as a human pathogen, and the murine pathogen Ehrlichia muris. Phylogenetic analysis supported the relationships among Ehrlichia species previously determined by comparison of 16S rRNA gene sequences. These sequences provide additional PCR targets for species for which few gene sequences have been determined. ...[more]

PMID: 10878077

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PCR amplification and comparison of nucleotide sequences from the groESL heat shock operon of Ehrlichia species.

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Predominance of Ehrlichia ewingii in Missouri dogs.

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Proposal to reclassify Ehrlichia muris as Ehrlichia muris subsp. muris subsp. nov. and description of Ehrlichia muris subsp. eauclairensis subsp. nov., a newly recognized tick-borne pathogen of humans.

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Ehrlichia ewingii infection in white-tailed deer (Odocoileus virginianus).

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Characterization and expression analysis of the groESL operon of Bartonella bacilliformis.

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Ehrlichia muris

Project description:Ehrlichia muris overview

| PRJNA232096 | ENA

Ehrlichia muris

Project description:Ehrlichia muris sequencing from Italy

| PRJNA1098473 | ENA

Persistent Ehrlichia ewingii infection in dogs after natural tick infestation.

Project description:BackgroundEhrlichia ewingii, which causes disease in dogs and people, is the most common Ehrlichia spp. infecting dogs in the United States, but little is known about how long E. ewingii infection persists in dogs.Hypothesis/objectivesTo evaluate the persistence of natural infection with E. ewingii in dogs.AnimalsFour Class A Beagles; no previous exposure to ticks or tick-borne infectious agents.MethodsDogs were exposed to ticks by weekly walks through tick habitat in north central Oklahoma; dogs positive for infection with Ehrlichia spp. by sequence-confirmed PCR and peptide-specific serology were evaluated for 733 days (D). Whole blood was collected once weekly for PCR, and serum was collected once monthly for detection of antibodies to Ehrlichia canis (peptide p16), Ehrlichia chaffeensis (indirect fluorescence antibody [IFA] and variable-length PCR target [VLPT]), and E. ewingii (peptide p28).ResultsAll dogs (4/4) became infected with Ehrlichia spp. as evidenced by seroconversion on IFA to E. chaffeensis (4/4); PCR detection of E. ewingii (4/4) and E. chaffeensis (2/4) DNA using both nested and real-time assays; and presence of specific antibodies to E. ewingii (4/4) and E. chaffeensis (2/4). Infection with E. chaffeensis was not detected after D55. Intermittent E. ewingii rickettsemia persisted in 3 of 4 dogs for as long as 733 days.Conclusions and clinical importanceOur data demonstrate that dogs infected with E. ewingii from tick feeding are capable of maintaining infection with this pathogen long-term, and may serve as a reservoir host for the maintenance of E. ewingii in nature.

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Prevalence of Ehrlichia ewingii in Amblyomma americanum in North Carolina.

Project description: Not available

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