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Fumonisin B₁ Inhibits Cell Proliferation and Decreases Barrier Function of Swine Umbilical Vein Endothelial Cells.

ABSTRACT: The fumonisins are a group of common mycotoxins found around the world that mainly contaminate maize. As environmental toxins, they pose a threat to human and animal health. Fumonisin B₁ (FB₁) is the most widely distributed and the most toxic. FB₁ can cause pulmonary edema in pigs. However, the current toxicity mechanism of fumonisins is still in the exploratory stage, which may be related to sphingolipid metabolism. Our study is designed to investigate the effect of FB₁ on the cell proliferation and barrier function of swine umbilical vein endothelial cells (SUVECs). We show that FB₁ can inhibit the cell viability of SUVECs. FB₁ prevents cells from entering the S phase from the G1 phase by regulating the expression of the cell cycle-related genes cyclin B1, cyclin D1, cyclin E1, Cdc25c, and the cyclin-dependent kinase-4 (CDK-4). This results in an inhibition of cell proliferation. In addition, FB₁ can also change the cell morphology, increase paracellular permeability, destroy tight junctions and the cytoskeleton, and reduce the expression of tight junction-related genes claudin 1, occludin, and ZO-1. This indicates that FB₁ can cause cell barrier dysfunction of SUVECs and promote the weakening or even destruction of the connections between endothelial cells. In turn, this leads to increased blood vessel permeability and promotes exudation. Our findings suggest that FB₁ induces toxicity in SUVECs by affecting cell proliferation and disrupting the barrier function.

SUBMITTER: Li Q

PROVIDER: S-EPMC8704807 | biostudies-literature |

REPOSITORIES: biostudies-literature

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