Dataset Information

Biodiversity of Root Endophytic Fungi from Oxyria sinensis Grown in Metal-Polluted and Unpolluted Soils in Yunnan Province, Southwestern China.

ABSTRACT: Oxyria sinensis adopts a tolerant strategy as a metal excluder to survive toxic metal concentrations. Biodiversity and the endophytic fungal community colonizing the O. sinensis roots were assessed from a mining area (MA) and a neighboring non-mining area (nMA) in southwestern China. All O. sinensis roots formed fully developed dark septate endophytes (DSEs) and arbuscular mycorrhizal fungi (AMF). Total DSE colonization was higher for the MA versus nMA, in contrast to the total AMF colonization in the two sites. The DSE colonization was higher than AMF colonization regardless of the site. Pure-culture data showed that the fungi closely related to Exophiala, Cadophora and Phialophora dominantly colonized the O. sinensis roots. A total of 450 operational taxonomic units (OTUs) were identified showing the presence of a distinct fungal community in MA and nMA, which was shaped by soil physiochemical properties, including soil Zn concentrations and organic matter. We found that O. sinensis accumulates and adapts efficiently to local endophytic fungi to achieve the expansion of its community, including the spontaneously reclaimed DSE. This property may be targeted to achieve its colonization with a pioneer plant for phytoremediation in the restoration of a vegetation cover in a metal-contaminated area.

SUBMITTER: Zhu M

PROVIDER: S-EPMC8705786 | biostudies-literature |

REPOSITORIES: biostudies-literature

ACCESS DATA

Similar Datasets

Project description:Epidemiological, ecological, and laboratory-based studies support the hypothesis that endocrine disrupting chemicals (EDCs) in the environment are responsible for developmental and reproductive abnormalities. We have previously described a killifish population resident in a highly polluted Superfund site (New Bedford Harbor, NBH) that shows evidence of exposure to an estrogenic environment and endocrine disruption. Here, we compare NBH with a local reference population (Scorton Creek, SC) for developmental patterns and direct effects of exogenous estradiol on the estrogenic markers, brain cytochrome P450 aromatase (CYP19A2 or AroB), hepatic vitellogenin (Vtg), and hepatic estrogen receptor alpha (ER alpha). In contrast to our previous observation of elevated ER alpha in NBH embryos, developmental levels of AroB and Vtg mRNAs did not differ between the two sites, demonstrating that not all estrogen-responsive genes are upregulated in NBH embryos. A dose-response experiment showed that NBH larvae are less responsive (lower maximum induction, as measured by ER alpha) and less sensitive (higher EC(50) for induction, as measured by AroB) to estradiol than SC larvae, changes that would be adaptive in an estrogenic environment. In contrast, induction of Vtg mRNA is similar in the two populations, indicating that the adaptive mechanism is target gene-specific. Based on the lower basal levels of ER alpha mRNA in several tissues from adult NBH fish vs SC fish (Greytak and Callard, 2007), we predicted estrogen hyporesponsiveness; however, induction of ER alpha by estradiol exposure in reproductively inactive males did not differ between the two sites. Moreover, AroB was more responsive and Vtg induction was greater (2d) or similar (5d) in NBH as compared to SC males. Worth noting is the high inter-individual variability in estrogen responses of gene targets, especially in NBH killifish, which may indicate evolving preadaptive or adaptive mechanisms. In conclusion, although multi-generational exposure to a highly polluted environment is associated with changes in basal levels of ER alpha mRNA, this is not a simple predictor of estrogen responsiveness. We hypothesize that adaptation of killifish to the estrogenic and polluted environment may be occurring through diverse mechanisms that are gene-, tissue type- and life-stage-specific.

Project description:The rapid developments in the next-generation sequencing methods in the recent years have provided a wealth of information on the community structures and functions of endophytic bacteria. However, the assembly processes of these communities in different plant tissues are still currently poorly understood, especially in wild plants in natural settings. The aim of this study was to compare the composition of endophytic bacterial communities in leaves and roots of arcto-alpine pioneer plant Oxyria digyna, and investigate, how plant tissue (leaf or root) or plant origin affect the community assembly. To address this, we planted micropropagated O. digyna plants with low bacterial load (bait plants) in experimental site with native O. digyna population, in the Low Arctic. The endophytic bacterial community structures in the leaves and roots of the bait plants were analyzed after one growing season and one year in the field, and compared to those of the wild plants growing at the same site. 16S rRNA gene targeted sequencing revealed that endophytic communities in the roots were more diverse than in the leaves, and the diversity in the bait plants increased in the field, and was highest in the wild plants. Both tissue type and plant group had strong impact on the endophytic bacterial community structures. Firmicutes were highly abundant in the leaf communities of both plant types. Proteobacteria and Bacteroidetes were more abundant in the roots, albeit with different relative abundances in different plant groups. The community structures in the bait plants changed in the field over time, and increasingly resembled the wild plant endophytic communities. This was due to the changes in the relative abundances of several bacterial taxa, as well as species acquisition in the field, but with no species turnover. Several OTUs that were acquired by the bait plants in the field and represent phosphate solubilizing and diazotrophic bacterial taxa, suggesting major role in nutrient acquisition of these bacteria for this nonmycorrhizal plant, thriving in the nutrient poor arctic soils.

Dataset Information

Biodiversity of Root Endophytic Fungi from Oxyria sinensis Grown in Metal-Polluted and Unpolluted Soils in Yunnan Province, Southwestern China.

Similar Datasets

OmicsDI is part of the ELIXIR infrastructure

Tweets