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Dysidenin from the Marine Sponge Citronia sp. Affects the Motility and Morphology of Haemonchus contortus Larvae In Vitro.

ABSTRACT: High-throughput screening of the NatureBank marine extract library (n = 7616) using a phenotypic assay for the parasitic nematode Haemonchus contortus identified an active extract derived from the Australian marine sponge Citronia sp. Bioassay-guided fractionation of the CH2Cl2/MeOH extract from Citronia sp. resulted in the purification of two known hexachlorinated peptides, dysidenin (1) and dysideathiazole (2). Compound 1 inhibited the growth/development of H. contortus larvae and induced multiple phenotypic changes, including a lethal evisceration (Evi) phenotype and/or somatic cell and tissue destruction. This is the first report of anthelmintic activity for these rare and unique polychlorinated peptides.

SUBMITTER: Ramage KS

PROVIDER: S-EPMC8708643 | biostudies-literature | 2021 Dec

REPOSITORIES: biostudies-literature

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Dysidenin from the Marine Sponge Citronia sp. Affects the Motility and Morphology of Haemonchus contortus Larvae In Vitro.

Ramage Kelsey S KS Taki Aya C AC Lum Kah Yean KY Hayes Sasha S Byrne Joseph J JJ Wang Tao T Hofmann Andreas A Ekins Merrick G MG White Jonathan M JM Jabbar Abdul A Davis Rohan A RA Gasser Robin B RB

Marine drugs 20211209 12

High-throughput screening of the NatureBank marine extract library (n = 7616) using a phenotypic assay for the parasitic nematode Haemonchus contortus identified an active extract derived from the Australian marine sponge Citronia sp. Bioassay-guided fractionation of the CH2Cl2/MeOH extract from Citronia sp. resulted in the purification of two known hexachlorinated peptides, dysidenin (1) and dysideathiazole (2). Compound 1 i ...[more]

PMID: 34940697

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