Unravelling the Initial Triggers of Botrytis cinerea Infection: First Description of Its Surfactome.

ABSTRACT: Botrytis cinerea is a critically important phytopathogenic fungus, causing devastating crop losses; signal transduction cascades mediate the "dialogue" among the fungus, plant, and environment. Surface proteins play important roles as front-line receptors. We report the first description of the surfactome of a filamentous fungus. To obtain a complete view of these cascades during infection of B. cinerea, its surfactome has been described by optimization of the "shaving" process and LC-MS/MS at two different infection stages, and with both rapid and late responses to environmental changes. The best results were obtained using PBS buffer in the "shaving" protocol. The surfactome obtained comprises 1010 identified proteins. These have been categorized by gene ontology and protei

SUBMITTER: Escobar-Nino A 

PROVIDER: S-EPMC8708654 | biostudies-literature | 2021 Nov

REPOSITORIES: biostudies-literature