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Fungal Communities in the Native New Zealand Medicinal Plant Pseudowintera colorata (Horopito) Are Determined by Plant Organ Type and Host Maturity with Key Members Promoting Plant Growth.

ABSTRACT: The plant Pseudowintera colorata is well known for its antimicrobial and medicinal properties and is endemic to New Zealand. Using PCR-Denaturing gradient gel electrophoresis (DGGE), we investigated the factors influencing the composition of endophytic fungal communities in P. colorata from ten distinct sites across New Zealand. Our results showed that plant organs of P. colorata influenced the diversity and richness of endophytic fungi (PERMANOVA, p < 0.05). In addition, plant maturity and its interactions revealed that endophytic fungal communities formed discrete clusters in leaves, stems, and roots of mature and immature P. colorata plants (PERMANOVA; p = 0.002, p = 0.001 and p = 0.039, respectively). For identifying isolates with biocontrol potential, dual culture tests were set up against four different phytopathogenic fungi. Isolates with high activity (zone of inhibition > 10 mm) were sequenced and identified as Trichoderma harzianum, Pezicula neosporulosa, Fusariumtricinctum, Metarhizium sp., and Chaetomium sp. Applying selected endophytic fungi (n = 7) as soil drenchers significantly increased the growth of P. colorata seedlings and produced more internodes. Seedling shoots treated with Trichoderma sp. PRY2BA21 were 2.2 × longer (8.36 cm) than the untreated controls (3.72 cm). Our results elucidate the main plant factors influencing fungal community composition and demonstrate a role for endophytic fungi in P. colorata growth and further demonstrate that medicinal plants are a rich source of endophytes with potential as biocontrol agents.

SUBMITTER: Purushotham N

PROVIDER: S-EPMC8709005 | biostudies-literature |

REPOSITORIES: biostudies-literature

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Project description:Endophytic mycopopulation isolated from India's Queen of herbs Tulsi (Ocimum sanctum) were explored and investigated for their diversity and antiphytopathogenic activity against widespread plant pathogens Botrytis cinerea, Sclerotinia sclerotiorum, Rhizoctonia solani and Fusarium oxysporum. 90 fungal isolates, representing 17 genera were recovered from 313 disease-free and surface sterilised plant segments (leaf and stem tissues) from three different geographic locations (Delhi, Hyderabad and Mukteshwar) during distinct sampling times in consequent years 2010 and 2011 in India. Fungal endophytes were subjected to molecular identification based on rDNA ITS sequence analysis. Plant pathogens such as F. verticillioides, B. maydis, C. coarctatum, R. bataticola, Hypoxylon sp., Diaporthe phaseolorum, Alternaria tenuissima and A. alternata have occurred as endophyte only during second sampling (second sampling in 2011) in the present study. Bi-plot generated by principal component analysis suggested tissue specificity of certain fungal endophytes. Dendrogram revealed species abundance as a function of mean temperature of the location at the time of sampling. Shannon diversity in the first collection is highest in Hyderabad leaf tissues (H' = 1.907) whereas in second collection it was highest from leaf tissues of Delhi (H' = 1.846). Mukteshwar (altitude: 7500 feet) reported least isolation rate in second collection. Nearly 23% of the total fungal isolates were considered as potent biocontrol agent. Hexane extract of M. phaseolina recovered from Hyderabad in first collection demonstrated highest activity against S. sclerotiorum with IC50 value of 0.38 mg/ml. Additionally, its components 2H-pyran-2-one, 5,6-dihydro-6-pentyl and palmitic acid, methyl ester as reported by GC-MS Chromatogram upon evaluation for their antiphytopathogenic activity exhibited IC50 value of 1.002 and 0.662 against respectively S. sclerotiorum indicating their significant role in antiphytopathogenic activity of hexane extract. The production of 2H-pyran-2-one, 5,6-dihydro-6-pentyl from M. phaseolina, an endophytic fungus is being reported for the first time.

Project description:BackgroundMedicinal plant species contribute significantly to folk medicine in Colombia. However, few local studies have investigated whether species used are introduced or native and whether there is a difference in importance of native and introduced medicinal plant species. The aim of the present study was to describe the use of medicinal plants within two municipalities, Campo Hermoso and Zetaquira, both in the department of Boyacá, Colombia and to assess the importance of native and introduced plants to healers, amateur healers and local people. As local healers including amateur healers have no history of introduced species our working hypotheses (H(1-2)) were that H(1): native and introduced medicinal plant species are of equal importance and H(2): healers and amateur healers do not differentiate in their preferences between native and introduced medicinal plant species.MethodsTen villages were included in the study. A combination of quantitative and qualitative methods was used including questionnaires, semi-structured interviews, in- depth interviews, and open talks. Voucher specimens were collected in home gardens and during field walks. For data analysis, we calculated use value indices and Jaccard index and tested for the above hypothesis using Spearman rank-correlation coefficients and Wilcoxon-Mann-Whitney tests.ResultsEighty medicinal plant species were described by locals as the most frequently used. Of these, 78 species were taxonomically identified, distributed within 41 families and 74 genera, which included 35 native species and 43 introduced. The highest valued families were: Asteraceae, Lamiaceae, Apiaceae, Rutaceae and Verbenaceae. The species ranked highest according to their Use Values, in both municipalities, were Mentha suaveolens Ehrh., Ambrosia cumanensis Kunth, and Verbena littoralis Kunth. Introduced species were more important than native ones in Zetaquira, while there was no difference in importance in Campo Hermoso. While healers relied most on the uses of native species, amateur healers were inclined to rely on introduced species. Medicinal plant administration in both municipalities follow the usual pattern: Leaves are used most commonly prepared by decoction or infusion and administrated orally.ConclusionsThe high proportion of introduced plant species used in the local traditional medicines is similar to the results of a number of other ethnobotanical studies and emphasise the need for efforts to record and maintain traditional knowledge on native species.

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Fungal Communities in the Native New Zealand Medicinal Plant Pseudowintera colorata (Horopito) Are Determined by Plant Organ Type and Host Maturity with Key Members Promoting Plant Growth.

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