Unknown

Dataset Information

0

Streamlined Subclass-Specific Absolute Quantification of Serum IgG Glycopeptides Using Synthetic Isotope-Labeled Standards.


ABSTRACT: Absolute glycoproteomics quantification has drawn tremendous attention owing to its prospects in biomarker discovery and clinical implementation but is impeded by a general lack of suitable heavy isotope-labeled glycopeptide standards. In this study, we devised a facile chemoenzymatic strategy to synthesize a total of 36 human IgG glycopeptides attached with well-defined glycoforms, including 15 isotope-labeled ones with a mass increment of 6 Da to their native counterparts. Spiking of these standards into human sera enabled simplified, robust, and precise absolute quantification of IgG glycopeptides in a subclass-specific fashion. Additionally, the implementation of the absolute quantification approach revealed subclass-dependent alteration of serum IgG galactosylation and sialylation in colon cancer samples.

SUBMITTER: Wang S 

PROVIDER: S-EPMC8715724 | biostudies-literature |

REPOSITORIES: biostudies-literature

Similar Datasets

| S-EPMC3579656 | biostudies-literature
2020-03-13 | PXD015025 | Pride
| S-EPMC4458369 | biostudies-literature
| S-EPMC6356711 | biostudies-literature
| S-EPMC8149832 | biostudies-literature
| S-EPMC6885709 | biostudies-literature
2009-10-27 | GSE15835 | GEO
| S-EPMC4180812 | biostudies-literature
2010-06-20 | E-GEOD-15835 | biostudies-arrayexpress
2023-06-15 | PXD043008 | JPOST Repository