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Fast Determination of a Novel Iron Chelate Prototype Used as a Fertilizer by Liquid Chromatography Coupled to a Diode Array Detector.

ABSTRACT: The environmental risk of the application of synthetic chelates has favored the implementation of new biodegradable ligands to correct Fe-deficient plants. This study developed and validated an analytical method for determination of a new prototype iron chelate─Fe(III)-benzeneacetate, 2-hydroxy-α-[(2-hydroxyethyl)amino]─(BHH/Fe³⁺) based on liquid chromatography with diode array detection, as a potential sustainable alternative. Chromatographic analysis was performed on a LiChrospher RP-18 in reverse-phase mode, with a mobile phase consisting of a mixture of acetonitrile (solvent A) and sodium borate buffer 0.20 mM at pH = 8 (solvent B) at a flow rate of 1.0 mL/min in isocratic elution mode. This method was fully validated and found to be linear from the limit of quantification (LOQ) to 50 mg/L and precise (standard deviation below 5%). The proposed method was demonstrated to be selective, precise, and robust. The developed methodology indicated that it is suitable for the quantification of iron chelate BHH/Fe³⁺.

SUBMITTER: Valverde S

PROVIDER: S-EPMC8719335 | biostudies-literature |

REPOSITORIES: biostudies-literature

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Phytochemical Characterization for Quality Control of Phyllostachys pubescens Leaves Using High-Performance Liquid Chromatography Coupled with Diode Array Detector and Tandem Mass Detector.

Project description:Phyllostachys pubescens leaves are cultivated in a number of Asian countries and have been used for antipyretic and diuretic effects since ancient times, especially in Korea. The purpose of this study was to develop and validate of analytical method for quality control of P. pubescens leaves using high-performance liquid chromatography with diode array detector (HPLC-DAD) and liquid chromatography with tandem mass spectrometry (LC-MS/MS) detection. HPLC-DAD analysis was conducted with a Gemini C18 column, and distilled water-acetonitrile (both with 0.1% (v/v) formic acid) mobile-phase system. For the LC-MS/MS analysis, all markers were separated with a Waters ACQUITY UPLC BEH C18 column and gradient flow system of distilled water containing 0.1% (v/v) formic acid and 5 mM ammonium formate-acetonitrile. In both method, major components were detected at 2.13-11.63 mg/g (HPLC-DAD) and 0.12-19.20 mg/g (LC-MS/MS). These methods were validated with respect to linearity (coefficient of determination >0.99), recovery (95.22-118.81%), accuracy (90.52-116.96), and precision (<4.0%), and were successfully applied for the quantitative analysis of P. pubescens leaves.

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Chemotaxonomic Monitoring of Genetically Authenticated Amomi Fructus Using High-Performance Liquid Chromatography-Diode Array Detector with Chemometric Analysis.

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| S-EPMC7583901 | biostudies-literature

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| S-EPMC9740684 | biostudies-literature

Separation and Quantification of Four Main Chiral Glucosinolates in <i>Radix Isatidis</i> and Its Granules Using High-Performance Liquid Chromatography/Diode Array Detector Coupled with Circular Dichroism Detection.

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| S-EPMC6374044 | biostudies-literature

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| S-EPMC6429096 | biostudies-literature

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| S-EPMC6972688 | biostudies-literature

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| S-EPMC8300601 | biostudies-literature

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