Spred2 controls the severity of Concanavalin A-induced liver damage by limiting interferon-gamma production by CD4⁺ and CD8⁺ T cells.

ABSTRACT:

Introduction

Mitogen-activated protein kinases (MAPKs) are involved in T cell-mediated liver damage. However, the inhibitory mechanism(s) that controls T cell-mediated liver damage remains unknown.

Objectives

We investigated whether Spred2 (Sprouty-related, EVH1 domain-containing protein 2) that negatively regulates ERK-MAPK pathway has a biological impact on T cell-mediated liver damage by using a murine model.

Methods

We induced hepatotoxicity in genetically engineered mice by intravenously injecting Concanavalin A (Con A) and analyzed the mechanisms using serum chemistry, histology, ELISA, qRT-PCR, Western blotting and flow cytometry.

Results

Spred2-deficient mice (Spred2^-/-) developed more sever liver damage than wild-type (WT) mice with increase

SUBMITTER: Sun C 

PROVIDER: S-EPMC8721245 | biostudies-literature | 2022 Jan

REPOSITORIES: biostudies-literature