Photoreceptor Degeneration in Homozygous Male Per2^luc Mice During Aging.

ABSTRACT: The Per2^luc mouse model developed by Takahashi laboratory is one of the most powerful models to study circadian rhythms in real time. In this study, we report that photoreceptors degenerate in male Per2^luc mice during aging. Young (2.5- to 5-month-old) and aged (11- to 13.5-month-old) homozygous male Per2^luc mice and C57BL/6J mice were used for this study. Retina structure and function were investigated via spectral domain optical coherence tomography (SD-OCT), fundus imaging, and electroretinography (ERG). Zonula occludens-1 (ZO-1) immunofluorescence was used to analyze the retinal pigment epithelium (RPE) morphology. Fundus examination revealed no difference between young Per2^luc and wild-type (WT) mice. However, the fundus of aged Per2^luc

SUBMITTER: Goyal V 

PROVIDER: S-EPMC8722430 | biostudies-literature | 2021 Apr

REPOSITORIES: biostudies-literature