Spectral image contrast-based flow digital nanoplasmon-metry for ultrasensitive antibody detection.

ABSTRACT:

Background

Gold nanoparticles (AuNPs) have been widely used in local surface plasmon resonance (LSPR) immunoassays for biomolecule sensing, which is primarily based on two conventional methods: absorption spectra analysis and colorimetry. The low figure of merit (FoM) of the LSPR and high-concentration AuNP requirement restrict their limit of detection (LOD), which is approximately ng to μg mL^-1 in antibody detection if there is no other signal or analyte amplification. Improvements in sensitivity have been slow in recent for a long time, and pushing the boundary of the current LOD is a great challenge of current LSPR immunoassays in biosensing.

Results

In this work, we developed spectral image contrast-based flow digital nanoplasmon-metry (Flow DiNM) to push the

SUBMITTER: Wang SH 

PROVIDER: S-EPMC8724237 | biostudies-literature | 2022 Jan

REPOSITORIES: biostudies-literature