Dataset Information

Endometrial microbiota composition is associated with reproductive outcome in infertile patients.

ABSTRACT:

Background

Previous evidence indicates associations between the female reproductive tract microbiome composition and reproductive outcome in infertile patients undergoing assisted reproduction. We aimed to determine whether the endometrial microbiota composition is associated with reproductive outcomes of live birth, biochemical pregnancy, clinical miscarriage or no pregnancy.

Methods

Here, we present a multicentre prospective observational study using 16S rRNA gene sequencing to analyse endometrial fluid and biopsy samples before embryo transfer in a cohort of 342 infertile patients asymptomatic for infection undergoing assisted reproductive treatments.

Results

A dysbiotic endometrial microbiota profile composed of Atopobium, Bifidobacterium, Chryseobacterium, Gardnerella, Haemophilus, Klebsiella, Neisseria, Staphylococcus and Streptococcus was associated with unsuccessful outcomes. In contrast, Lactobacillus was consistently enriched in patients with live birth outcomes.

Conclusions

Our findings indicate that endometrial microbiota composition before embryo transfer is a useful biomarker to predict reproductive outcome, offering an opportunity to further improve diagnosis and treatment strategies. Video Abstract.

SUBMITTER: Moreno I

PROVIDER: S-EPMC8725275 | biostudies-literature | 2022 Jan

REPOSITORIES: biostudies-literature

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Publications

Endometrial microbiota composition is associated with reproductive outcome in infertile patients.

Moreno Inmaculada I Garcia-Grau Iolanda I Perez-Villaroya David D Gonzalez-Monfort Marta M Bahçeci Mustafa M Barrionuevo Marcelo J MJ Taguchi Sagiri S Puente Elena E Dimattina Michael M Lim Mei Wei MW Meneghini Georgina G Aubuchon Mira M Leondires Mark M Izquierdo Alexandra A Perez-Olgiati Martina M Chavez Alejandro A Seethram Ken K Bau Davide D Gomez Carlos C Valbuena Diana D Vilella Felipe F Simon Carlos C

Microbiome 20220104 1

<h4>Background</h4>Previous evidence indicates associations between the female reproductive tract microbiome composition and reproductive outcome in infertile patients undergoing assisted reproduction. We aimed to determine whether the endometrial microbiota composition is associated with reproductive outcomes of live birth, biochemical pregnancy, clinical miscarriage or no pregnancy.<h4>Methods</h4>Here, we present a multicentre prospective observational study using 16S rRNA gene sequencing to ...[more]

PMID: 34980280

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Project description:BackgroundThe previous researches show that infertile patients have a higher incidence of endometritis and endometrial polyps, and the occurrence of these two diseases is related to changes in the microbiota of the genital tract. We aim to determine the composition and changing characteristics of the microbiota in the genital tract (especially the endometrium) of infertile patients with chronic endometritis or endometrial polyps, and find the correlation between it and the occurrence of diseases.MethodsThis is a prospective study. We collected genital tract biopsy samples from 134 asymptomatic infertile patients receiving assisted reproductive therapy before embryo transfer. Through pathological examination and 16S ribosomal RNA(16S rRNA) sequencing, we determined the distribution of chronic endometritis and endometrial polyps in these patients, as well as their distribution of reproductive tract microorganisms.ResultsCompared with the normal control group, the microbial group of reproductive tract in patients with chronic endometritis and endometrial polyps is changed, and there are significant species differences and relative abundance differences in the vagina, cervix and uterine cavity. Lactobacillus, the dominant flora of female genital tract, showed a change in abundance in patients with endometrial diseases. Endometrial microbiota composed of Staphylococcus, Gardnerella, Atopobium, Streptococcus, Peptostreptococcus, Chlamydia, Fusobacterium, Acinetobacter, etc. are related to chronic endometritis and endometrial polyps.ConclusionThe results showed that, compared with the normal control group, the endometrial microbiota of infertile patients with chronic endometritis or endometrial polyps did have significant changes in the relative abundance distribution of species, suggesting that changes in local microecology may be an important factor in the occurrence of disease, or even adverse pregnancy outcomes. The further study of endometrial microecology may provide a new opportunity to further improve the diagnosis and treatment strategy of chronic endometritis.

Project description:PurposeThe uterine immunophenotype is relatively poorly understood, with most studies reporting proportions/percentages. A novel technique to calculate local endometrial lymphocyte concentrations is described, and used to compare results between aetiological subgroups such as repeated implantation failure (RIF) and recurrent pregnancy loss (RPL) with male-factor controls.Methods455 patients had an endometrial biopsy performed. Background history on initial presentation was used to subdivide the population into RIF (n?=?149), RPL (n?=?121), primary (n?=?76) and secondary infertility (n?=?80). A control group was identified comprising male factor infertility aetiology with all female investigations normal (n?=?29). Endometrial Tissue was assessed using a comprehensive multi-parameter panel. Lymphocyte subpopulations were calculated using flowcount flurospheres and a mathematical correction applied to determine concentrations per milligram of tissue, based on original biopsy weight and volumetric dilutions.ResultsThe flow cytometry technique was successful in determining population centiles for concentrations of endometrial lymphocyte subsets. Distinct differences were noted across the patient groups. Th2 concentrations were significantly higher in the controls (p?=?0.0002). All RPL/infertile populations had increased concentrations of peripheral type NK's (p?=?0.016) and B cells (p?=?0.045). Relative to male factor controls, CD4+ and CD8+ T lymphocyte populations were increased in RPL patients, and reduced in those with a history of RIF. Th1 concentrations were elevated in the adverse outcome groups (p?=?0.032). Concentration centiles alone do not appear to accurately predict outcome with subsequent treatment.ConclusionsEndometrial biopsy analysis by flow cytometry can provide detailed analysis of constituent lymphocyte subsets by concentration as well as proportion. This novel approach provides additional independent data to further assess the significance of endometrial changes in the setting of reproductive failure.

Dataset Information

Endometrial microbiota composition is associated with reproductive outcome in infertile patients.

Background

Methods

Results

Conclusions

Publications

Endometrial microbiota composition is associated with reproductive outcome in infertile patients.

Similar Datasets

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