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Electrospun multifunctional nanofibrous mats loaded with bioactive anemoside B4 for accelerated wound healing in diabetic mice.

ABSTRACT: With the worldwide prevalence of diabetes and considering the complicated microenvironment of diabetic wounds, the design and development of innovative multifunctional wound dressing materials are much wanted for the treatment of hard-to-heal wounds in diabetic patients. In the present study, anti-inflammatory ingredients loaded with nanofibrous wound dressing materials were manufactured by a promising blend-electrospinning strategy, and their capability for treating the diabetic wound was also systematically explored. A polymer blend consisting of Chitosan (CS) and polyvinyl alcohol (PVA) was electrospun into CS-PVA nanofibrous mats as control groups. In the meanwhile, a bioactive ingredient of Chinese medicine Pulsatilla, anemoside B4(ANE), with different contents were loaded into the electrospinning solution to construct CS-PVA-ANE nanofibrous mats. The developed CS-PVA-ANE wound dressing materials exhibited multifunctional properties including prominent water absorption, biomimetic elastic mechanical properties, and sustained ANE releasing behavior, as well as outstanding hemostatic properties. The in vitro studies showed that the CS-PVA-ANE nanofiber mats could significantly suppress lipopolysaccharide (LPS)-stimulated differentiation of pro-inflammatory (M1) macrophage subsets, and notably reduce the reactive oxygen species (ROS) generation, as well as obviously decrease inflammatory cytokine release. The in vivo animal studies showed that the CS-PVA-ANE nanofiber mats promoted the healing of diabetic wounds by significantly enhancing wound closure rates, accelerating excellent angiogenesis, promoting re-epithelization and collagen matrix deposition throughout all stages of wound healing. The present study demonstrated that CS-PVA-ANE nanofiber mats could effectively shorten the wound-healing time by inhibiting inflammatory activity, which makes them promising candidates for the treatment of hard-to-heal wounds caused by diabetes.

SUBMITTER: Zhang H

PROVIDER: S-EPMC8725929 | biostudies-literature |

REPOSITORIES: biostudies-literature

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Project description:The fabrication of polymeric nanofibers and its potential versatility instigated to foster smart hybrid nanomaterials for the removal of environmental pollutants. In this pursuit, in this research work, polyacrylonitrile (PAN)-based two-dimensional (2D) nanofibrous mats with polyethyleneimine (PEI)/Fe and quaternary ammonium (QA)/Fe as hybrid fillers were prepared by the electrospinning process for the effective dye removal and bacterial disinfection. The characteristics of the fabricated nanomaterials were extensively explored by several analytical techniques such as field emission-scanning electron microscopy, transmission electron microscopy, X-ray diffraction, Fourier transform infrared spectroscopy, and Brunauer-Emmett-Teller analysis. Magnetic and thermal properties were investigated by superconducting quantum interference device and thermogravimetric measurements. The kinetic and isothermal models affirmed the adsorption behavior of the PAN-PEI/Fe nanofibers, and further regenerative studies substantiated the sustainability of the mats for the removal of industrial dye effluents. Subsequently, the magnetic-QA-loaded PAN nanofiber mats exhibited bactericidal killing efficacy of 99 and 89.5% in both Staphylococcus aureus and green fluorescence protein expressing Escherichia coli bacterial models evaluated from the conventional quantitative bacterial colony-counting assay. Disk diffusion method and microscopic investigations corroborated the disinfection efficacy with zone of inhibitions of ∼23 and 33 mm, respectively. Interestingly, in vitro cell culture studies conducted in BHK-21 and NIH 3T3 cell lines demonstrated the cytocompatibility, and the in vivo toxicity investigations using the zebrafish models necessitated the real-time application of these nanofibrous mats. Therefore, the comprehensive study of the fabricated PAN-templated functionalized 2D nanofibrous mats affirmed to be competent for the remediation of industrial dye effluents and bacteria in water bodies.

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Electrospun multifunctional nanofibrous mats loaded with bioactive anemoside B4 for accelerated wound healing in diabetic mice.

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