Mitotic Activation Around Wound Edges and Epithelialization Repair in UVB-Induced Capsular Cataracts.

ABSTRACT:

Purpose

Ultraviolet B (UVB) has been well documented to induce capsular cataracts; however, the mechanism of the lens epithelial cell-mediated repair process after UVB irradiation is not fully understood. The purpose of this study was to better understand lens epithelial cell repair after UVB-induced epithelium damage.

Method

C57BL/6J mice were irradiated by various doses of UVB. Lens morphology and lens capsule opacity were monitored by slit lamp, darkfield microscopy, and phase-contrast microscopy. Lens epithelial cell mitotic activation and cell apoptosis were measured by immunohistochemistry. Lens epithelial ultrastructure was analyzed by transmission electron microscopy.

Results

UVB irradiation above a dose of 2.87 kJ/m2 triggered lens epithelial cell apoptosis a

SUBMITTER: Wei Z 

PROVIDER: S-EPMC8727316 | biostudies-literature | 2021 Dec

REPOSITORIES: biostudies-literature