EFAMIX, a tool to decompose inline chromatography SAXS data from partially overlapping components.

ABSTRACT: Small-angle X-ray scattering (SAXS) is an established technique for structural analysis of biological macromolecules in solution. During the last decade, inline chromatography setups coupling SAXS with size exclusion (SEC-SAXS) or ion exchange (IEC-SAXS) have become popular in the community. These setups allow one to separate individual components in the sample and to record SAXS data from isolated fractions, which is extremely important for subsequent data interpretation, analysis, and structural modeling. However, in case of partially overlapping elution peaks, inline chromatography SAXS may still yield scattering profiles from mixtures of components. The deconvolution of these scattering data into the individual fractions is nontrivial and potentially ambiguous. We describe a cross-plat

SUBMITTER: Konarev PV 

PROVIDER: S-EPMC8740826 | biostudies-literature | 2022 Jan

REPOSITORIES: biostudies-literature