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Single-cell architecture and functional requirement of alternative splicing during hematopoietic stem cell formation.

ABSTRACT: [Figure: see text].

SUBMITTER: Wang F

PROVIDER: S-EPMC8741192 | biostudies-literature | 2022 Jan

REPOSITORIES: biostudies-literature

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Single-cell architecture and functional requirement of alternative splicing during hematopoietic stem cell formation.

Wang Fang F Tan Puwen P Zhang Pengcheng P Ren Yue Y Zhou Jie J Li Yunqiao Y Hou Siyuan S Li Shuaili S Zhang Linlin L Ma Yanni Y Wang Chaojie C Tang Wanbo W Wang Xiaoshuang X Huo Yue Y Hu Yongfei Y Cui Tianyu T Niu Chao C Wang Dong D Liu Bing B Lan Yu Y Yu Jia J

Science advances 20220107 1

Single-cell transcriptional profiling has rapidly advanced our understanding of the embryonic hematopoiesis; however, whether and what role RNA alternative splicing (AS) plays remains an enigma. This is important for understanding the mechanisms underlying splicing-associated hematopoietic diseases and for the derivation of therapeutic stem cells. Here, we used single-cell full-length transcriptome data to construct an isoform-based transcriptional atlas of the murine endothelial-to-hematopoieti ...[more]

PMID: 34995116

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