Ontology highlight
ABSTRACT:
SUBMITTER: Liebl MP
PROVIDER: S-EPMC8742033 | biostudies-literature | 2022 Jan
REPOSITORIES: biostudies-literature
Liebl Martina P MP Meister Sarah C SC Frey Lisa L Hendrich Kristina K Klemmer Anja A Wohlfart Bettina B Untucht Christopher C Nuber Judith J Pohl Christian C Lakics Viktor V
Scientific reports 20220107 1
Autophagic flux can be quantified based on the accumulation of lipidated LC3B in the presence of late-stage autophagy inhibitors. This method has been widely applied to identify novel compounds that activate autophagy. Here we scrutinize this approach and show that bafilomycin A1 (BafA) but not chloroquine is suitable for flux quantification due to the stimulating effect of chloroquine on non-canonical LC3B-lipidation. Significant autophagic flux increase by rapamycin could only be observed when ...[more]