Ontology highlight
ABSTRACT:
SUBMITTER: Yan B
PROVIDER: S-EPMC8744680 | biostudies-literature | 2022 Jan
REPOSITORIES: biostudies-literature
Yan Bo B Tzertzinis George G Schildkraut Ira I Ettwiller Laurence L
Genome research 20211123 1
Determination of eukaryotic transcription start sites (TSSs) has been based on methods that require the cap structure at the 5' end of transcripts derived from Pol II RNA polymerase. Consequently, these methods do not reveal TSSs derived from the other RNA polymerases that also play critical roles in various cell functions. To address this limitation, we developed ReCappable-seq, which comprehensively identifies TSS for both Pol II and non-Pol II transcripts at single-nucleotide resolution. The ...[more]