Unknown

Dataset Information

0

STOP1 Regulates LKS1 Transcription and Coordinates K+/NH4+ Balance in Arabidopsis Response to Low-K+ Stress.


ABSTRACT: Potassium and nitrogen are essential mineral elements for plant growth and development. The protein kinase LKS1/CIPK23 is involved in both K+ and NH4+ uptake in Arabidopsis root. The transcripts of LKS1 can be induced by low K+ (0.1 mM) and high NH4+ (30 mM); however, the molecular mechanism is still unknown. In this study, we isolated the transcription factor STOP1 that positively regulates LKS1 transcription in Arabidopsis responses to both low-K+ and high-NH4+ stresses. STOP1 proteins can directly bind to the LKS1 promoter, promoting its transcription. The stop1 mutants displayed a leaf chlorosis phenotype similar to lks1 mutant when grown on low-K+ and high-NH4+ medium. On the other hand, STOP1 overexpressing plants exhibited a similar tolerant phenotype to LKS1 overexpressing plants. The transcript level of STOP1 was only upregulated by low K+ rather than high NH4+; however, the accumulation of STOP1 protein in the nucleus was required for the upregulation of LKS1 transcripts in both low-K+ and high-NH4+ responses. Our data demonstrate that STOP1 positively regulates LKS1 transcription under low-K+ and high-NH4+ conditions; therefore, LKS1 promotes K+ uptake and inhibits NH4+ uptake. The STOP1/LKS1 pathway plays crucial roles in K+ and NH4+ homeostasis, which coordinates potassium and nitrogen balance in plants in response to external fluctuating nutrient levels.

SUBMITTER: Wang ZF 

PROVIDER: S-EPMC8745191 | biostudies-literature | 2021 Dec

REPOSITORIES: biostudies-literature

altmetric image

Publications

STOP1 Regulates <i>LKS1</i> Transcription and Coordinates K<sup>+</sup>/NH<sub>4</sub><sup>+</sup> Balance in <i>Arabidopsis</i> Response to Low-K<sup>+</sup> Stress.

Wang Zhi-Fang ZF   Mi Ting-Wei TW   Gao Yong-Qiang YQ   Feng Han-Qian HQ   Wu Wei-Hua WH   Wang Yi Y  

International journal of molecular sciences 20211229 1


Potassium and nitrogen are essential mineral elements for plant growth and development. The protein kinase LKS1/CIPK23 is involved in both K<sup>+</sup> and NH<sub>4</sub><sup>+</sup> uptake in <i>Arabidopsis</i> root. The transcripts of <i>LKS1</i> can be induced by low K<sup>+</sup> (0.1 mM) and high NH<sub>4</sub><sup>+</sup> (30 mM); however, the molecular mechanism is still unknown. In this study, we isolated the transcription factor STOP1 that positively regulates <i>LKS1</i> transcription  ...[more]

Similar Datasets

| S-EPMC6320511 | biostudies-literature
| S-EPMC11282296 | biostudies-literature
| S-EPMC1899371 | biostudies-literature
| S-EPMC8507492 | biostudies-literature
| S-EPMC555978 | biostudies-other
| S-EPMC8719490 | biostudies-literature
| S-EPMC7194377 | biostudies-literature
2013-10-21 | GSE45017 | GEO
| S-EPMC4092073 | biostudies-literature
| S-EPMC11371176 | biostudies-literature