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Phiclust: a clusterability measure for single-cell transcriptomics reveals phenotypic subpopulations.


ABSTRACT: The ability to discover new cell phenotypes by unsupervised clustering of single-cell transcriptomes has revolutionized biology. Currently, there is no principled way to decide whether a cluster of cells contains meaningful subpopulations that should be further resolved. Here, we present phiclust (ϕclust), a clusterability measure derived from random matrix theory that can be used to identify cell clusters with non-random substructure, testably leading to the discovery of previously overlooked phenotypes.

SUBMITTER: Mircea M 

PROVIDER: S-EPMC8751334 | biostudies-literature |

REPOSITORIES: biostudies-literature

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