Unknown

Dataset Information

0

Rapid preimplantation genetic screening using a handheld, nanopore-based DNA sequencer.


ABSTRACT:

Objective

To determine if a handheld, nanopore-based DNA sequencer can be used for rapid preimplantation genetic screening (PGS).

Design

Laboratory study.

Setting

Academic medical center.

Patient(s)

Amplified genomic DNA from euploid and aneuploid trophectoderm biopsy samples (n=9) that was also tested using traditional next generation sequencing (NGS).

Intervention(s)

Short-read DNA library preparation and nanopore-based sequencing using a hand-held MinION sequencer.

Main outcome measure(s)

Comparison of cytogenetic testing result from NGS and nanopore-based sequencing and the time required for library preparation and sequencing.

Result(s)

Multiplexed short-read DNA library preparation was completed in 45 minutes. Sequencing on a single sample was completed within 20 minutes and 5 samples were simultaneously sequenced in under 2 hours. Whole-chromosome aneuploidy screening results obtained from nanopore-based sequencing were identical to those obtained using NGS.

Conclusion(s)

Here we report the first application of nanopore-based sequencing for PGS on trophectoderm biopsy samples using a novel rapid multiplxed short-read nanopore sequencing library preparation protocol. Sequencing for aneuploidy screening could be performed on a single sample in 20 minutes and on 5 samples, simultaneously, within 2 hours. Overall, nanopore sequencing is a promising tool to perform rapid PGS onsite, enabling same day testing and embryo transfer, thus obviating the need for complex, large and expensive DNA sequencers or embryo freezing.

SUBMITTER: Wei S 

PROVIDER: S-EPMC8756381 | biostudies-literature |

REPOSITORIES: biostudies-literature

Similar Datasets

| S-EPMC7873225 | biostudies-literature
| S-EPMC5516037 | biostudies-literature
| S-EPMC7002467 | biostudies-literature
| S-EPMC6396348 | biostudies-literature
2023-09-04 | GSE241410 | GEO
| S-EPMC3240684 | biostudies-literature
| S-EPMC4326468 | biostudies-literature
| S-EPMC6318931 | biostudies-literature
| S-EPMC4907500 | biostudies-literature
| S-EPMC7671332 | biostudies-literature