Ontology highlight
ABSTRACT: Objective
To determine if a handheld, nanopore-based DNA sequencer can be used for rapid preimplantation genetic screening (PGS).Design
Laboratory study.Setting
Academic medical center.Patient(s)
Amplified genomic DNA from euploid and aneuploid trophectoderm biopsy samples (n=9) that was also tested using traditional next generation sequencing (NGS).Intervention(s)
Short-read DNA library preparation and nanopore-based sequencing using a hand-held MinION sequencer.Main outcome measure(s)
Comparison of cytogenetic testing result from NGS and nanopore-based sequencing and the time required for library preparation and sequencing.Result(s)
Multiplexed short-read DNA library preparation was completed in 45 minutes. Sequencing on a single sample was completed within 20 minutes and 5 samples were simultaneously sequenced in under 2 hours. Whole-chromosome aneuploidy screening results obtained from nanopore-based sequencing were identical to those obtained using NGS.Conclusion(s)
Here we report the first application of nanopore-based sequencing for PGS on trophectoderm biopsy samples using a novel rapid multiplxed short-read nanopore sequencing library preparation protocol. Sequencing for aneuploidy screening could be performed on a single sample in 20 minutes and on 5 samples, simultaneously, within 2 hours. Overall, nanopore sequencing is a promising tool to perform rapid PGS onsite, enabling same day testing and embryo transfer, thus obviating the need for complex, large and expensive DNA sequencers or embryo freezing.
SUBMITTER: Wei S
PROVIDER: S-EPMC8756381 | biostudies-literature |
REPOSITORIES: biostudies-literature