MAL2 mediates the formation of stable HER2 signaling complexes within lipid raft-rich membrane protrusions in breast cancer cells.
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ABSTRACT:
SUBMITTER: Jeong J
PROVIDER: S-EPMC8762588 | biostudies-literature |
REPOSITORIES: biostudies-literature
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Project description:The lipid raft resident protein, MAL2, has been implicated as contributing to the pathogenesis of several malignancies, including breast cancer, but the underlying mechanism for its effects on tumorigenesis is unknown. Here, we show that MAL2-mediated lipid raft formation leads to HER2 plasma membrane retention and enhanced HER2 signaling in breast cancer cells. We employed proximity ligation assays (PLA) to define physical interactions between HER2 and MAL2 in lipid rafts and super-resolution structured illumination microscopy (SR-SIM imaging) to determine the structural organization of the HER2/Ezrin/NHERF1/PMCA2 protein complex. Formation of this protein complex maintains low intracellular calcium concentrations in the vicinity of the plasma membrane. Importantly, we also observed enhanced HER2-MAL2 protein interactions in lipid rafts in trastuzumab-resistant breast cancer cells. Our findings suggest that MAL2 is crucial for lipid raft formation, HER2 signaling and HER2 membrane stability in breast cancer cells, suggesting MAL2 as a potential therapeutic target.
2021-12-15 | GSE188513 | GEO
Project description:MAL2 mediates the formation of stable HER2 signaling complexes within lipid raft-rich membrane protrusions in breast cancer cells.
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