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Genomic and phylogenetic analysis of hepatitis C virus isolates from argentine patients: a six-year retrospective study.

ABSTRACT: Typing of hepatitis C virus (HCV) isolates from Argentine patients was performed by using different methodologies in a population of 243 patients. HCV subtype was assigned based upon restriction fragment length polymorphism (RFLP). HCV RNA genomes obtained from serum samples were classified as belonging to clade 1 (53.5%), 2 (23. 0%), or 3 (8.6%); 14.8% of samples showed HCV mixed infections, more frequently implying different subtypes within the same clade. In addition to RFLP typing, phylogenetic relatedness among sequences from both 5' untranslated region (n = 50) and nonstructural 5B coding region (n = 15) was established.

SUBMITTER: Quarleri JF

PROVIDER: S-EPMC87637 | biostudies-literature | 2000 Dec

REPOSITORIES: biostudies-literature

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Genomic and phylogenetic analysis of hepatitis C virus isolates from argentine patients: a six-year retrospective study.

Quarleri J F JF Robertson B H BH Mathet V L VL Feld M M Espínola L L Requeijo M P MP Mandó O O Carballal G G Oubiña J R JR

Journal of clinical microbiology 20001201 12

Typing of hepatitis C virus (HCV) isolates from Argentine patients was performed by using different methodologies in a population of 243 patients. HCV subtype was assigned based upon restriction fragment length polymorphism (RFLP). HCV RNA genomes obtained from serum samples were classified as belonging to clade 1 (53.5%), 2 (23. 0%), or 3 (8.6%); 14.8% of samples showed HCV mixed infections, more frequently implying different subtypes within the same clade. In addition to RFLP typing, phylogene ...[more]

PMID: 11101596

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