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Toward Multiplexed Optogenetic Circuits.

ABSTRACT: Owing to its ubiquity and easy availability in nature, light has been widely employed to control complex cellular behaviors. Light-sensitive proteins are the foundation to such diverse and multilevel adaptive regulations in a large range of organisms. Due to their remarkable properties and potential applications in engineered systems, exploration and engineering of natural light-sensitive proteins have significantly contributed to expand optogenetic toolboxes with tailor-made performances in synthetic genetic circuits. Progressively, more complex systems have been designed in which multiple photoreceptors, each sensing its dedicated wavelength, are combined to simultaneously coordinate cellular responses in a single cell. In this review, we highlight recent works and challenges on multiplexed optogenetic circuits in natural and engineered systems for a dynamic regulation breakthrough in biotechnological applications.

SUBMITTER: Dwijayanti A

PROVIDER: S-EPMC8766309 | biostudies-literature | 2021

REPOSITORIES: biostudies-literature

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Toward Multiplexed Optogenetic Circuits.

Dwijayanti Ari A Zhang Congqiang C Poh Chueh Loo CL Lautier Thomas T

Frontiers in bioengineering and biotechnology 20220105

Owing to its ubiquity and easy availability in nature, light has been widely employed to control complex cellular behaviors. Light-sensitive proteins are the foundation to such diverse and multilevel adaptive regulations in a large range of organisms. Due to their remarkable properties and potential applications in engineered systems, exploration and engineering of natural light-sensitive proteins have significantly contributed to expand optogenetic toolboxes with tailor-made performances in syn ...[more]

PMID: 35071213

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