Dataset Information

Accurate determination of marker location within whole-brain microscopy images.

ABSTRACT: High-resolution whole-brain microscopy provides a means for post hoc determination of the location of implanted devices and labelled cell populations that are necessary to interpret in vivo experiments designed to understand brain function. Here we have developed two plugins (brainreg and brainreg-segment) for the Python-based image viewer napari, to accurately map any object in a common coordinate space. We analysed the position of dye-labelled electrode tracks and two-photon imaged cell populations expressing fluorescent proteins. The precise location of probes and cells were physiologically interrogated and revealed accurate segmentation with near-cellular resolution.

SUBMITTER: Tyson AL

PROVIDER: S-EPMC8766598 | biostudies-literature | 2022 Jan

REPOSITORIES: biostudies-literature

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Accurate determination of marker location within whole-brain microscopy images.

Tyson Adam L AL Vélez-Fort Mateo M Rousseau Charly V CV Cossell Lee L Tsitoura Chryssanthi C Lenzi Stephen C SC Obenhaus Horst A HA Claudi Federico F Branco Tiago T Margrie Troy W TW

Scientific reports 20220118 1

High-resolution whole-brain microscopy provides a means for post hoc determination of the location of implanted devices and labelled cell populations that are necessary to interpret in vivo experiments designed to understand brain function. Here we have developed two plugins (brainreg and brainreg-segment) for the Python-based image viewer napari, to accurately map any object in a common coordinate space. We analysed the position of dye-labelled electrode tracks and two-photon imaged cell popula ...[more]

PMID: 35042882

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Dataset Information

Accurate determination of marker location within whole-brain microscopy images.

Publications

Accurate determination of marker location within whole-brain microscopy images.

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