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Isolation of Legionella anisa using an amoebic coculture procedure.

ABSTRACT: Conventional diagnostic tests for legionellosis were negative for a 61-year-old immunocompromised man with pneumonia. However, coculture of a sputum sample with Acanthamoeba polyphaga amoebae led to the recovery of Legionella anisa. This procedure may be a sensitive and convenient diagnostic method, especially for non-Legionella pneumophila species infections that can be diagnosed only by culture.

SUBMITTER: La Scola B

PROVIDER: S-EPMC87733 | biostudies-literature | 2001 Jan

REPOSITORIES: biostudies-literature

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Isolation of Legionella anisa using an amoebic coculture procedure.

La Scola B B Mezi L L Weiller P J PJ Raoult D D

Journal of clinical microbiology 20010101 1

Conventional diagnostic tests for legionellosis were negative for a 61-year-old immunocompromised man with pneumonia. However, coculture of a sputum sample with Acanthamoeba polyphaga amoebae led to the recovery of Legionella anisa. This procedure may be a sensitive and convenient diagnostic method, especially for non-Legionella pneumophila species infections that can be diagnosed only by culture. ...[more]

PMID: 11136802

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