Dataset Information

A Novel WRKY Transcription Factor from Ipomoea trifida, ItfWRKY70, Confers Drought Tolerance in Sweet Potato.

ABSTRACT: WRKY transcription factors are one of the important families in plants, and have important roles in plant growth, abiotic stress responses, and defense regulation. In this study, we isolated a WRKY gene, ItfWRKY70, from the wild relative of sweet potato Ipomoea trifida (H.B.K.) G. Don. This gene was highly expressed in leaf tissue and strongly induced by 20% PEG6000 and 100 μM abscisic acid (ABA). Subcellar localization analyses indicated that ItfWRKY70 was localized in the nucleus. Overexpression of ItfWRKY70 significantly increased drought tolerance in transgenic sweet potato plants. The content of ABA and proline, and the activity of SOD and POD were significantly increased, whereas the content of malondialdehyde (MDA) and H2O2 were decreased in transgenic plants under drought stress. Overexpression of ItfWRKY70 up-regulated the genes involved in ABA biosynthesis, stress-response, ROS-scavenging system, and stomatal aperture in transgenic plants under drought stress. Taken together, these results demonstrated that ItfWRKY70 plays a positive role in drought tolerance by accumulating the content of ABA, regulating stomatal aperture and activating the ROS scavenging system in sweet potato.

SUBMITTER: Sun S

PROVIDER: S-EPMC8775875 | biostudies-literature | 2022 Jan

REPOSITORIES: biostudies-literature

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A Novel WRKY Transcription Factor from Ipomoea trifida, ItfWRKY70, Confers Drought Tolerance in Sweet Potato.

Sun Sifan S Li Xu X Gao Shaopei S Nie Nan N Zhang Huan H Yang Yufeng Y He Shaozhen S Liu Qingchang Q Zhai Hong H

International journal of molecular sciences 20220108 2

WRKY transcription factors are one of the important families in plants, and have important roles in plant growth, abiotic stress responses, and defense regulation. In this study, we isolated a WRKY gene, ItfWRKY70, from the wild relative of sweet potato Ipomoea trifida (H.B.K.) G. Don. This gene was highly expressed in leaf tissue and strongly induced by 20% PEG6000 and 100 μM abscisic acid (ABA). Subcellar localization analyses indicated that ItfWRKY70 was localized in the nucleus ...[more]

PMID: 35054868

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Project description:BackgroundWRKY transcription factors are plant-specific and play essential roles in growth, development, and stress responses, including reactions to salt, drought, and cold. Despite their significance, the WRKY genes in the wild sweet potato ancestor, Ipomoea pes-caprae, remain unexplored.ResultsIn this study, 65 WRKY genes were identified in the I. pes-caprae transcriptomic data. A phylogenetic tree incorporating Arabidopsis thaliana and Ipomoea batatas revealed seven major groups, each characterized by conserved gene structural features. Transcriptome data of I. pes-caprae under salt stress conditions identified 17 highly expressed WRKY genes, whose promoter regions contain cis-acting elements associated with plant growth, stress responses, and hormone signaling. Further analysis revealed that the 17 IpWRKY genes exhibited differential expression patterns under various abiotic stresses, suggesting their potential roles in specific stress responses. The gene IpWRKY16 was significantly up-expressed under salt stress, drought, salicylic acid (SA), and abscisic acid (ABA) treatments. Subcellular localization analysis confirmed that IpWRKY16 is located in the nucleus. Under salt stress, IpWRKY16 overexpressing roots showed high activity in superoxide dismutase (SOD), peroxidase (POD), catalase (CAT), and low content in malondialdehyde (MDA). Using non-invasive micro-test technology (NMT), a significant efflux of Na+ was observed in the elongation zones of sweet potato adventitious roots that overexpressed IpWRKY16. Quantitative reverse transcription PCR (qRT-PCR) revealed that several ion transporter genes were responsive to IpWRKY16 expression, with IbSOS3, IbAHA4-1, and IbAHA4-2 showing the highest expression levels. We hypothesize that IpWRKY16 responds to salt stress by forming a complex regulatory network involving these key genes.ConclusionsThis study provides a foundational understanding of WRKY transcription factors in I. pes-caprae, offering insights into their potential role in enhancing salt-tolerance in sweet potato. Our findings contribute valuable genetic knowledge that could aid in the molecular breeding of stress-resilient sweet potato varieties.

Dataset Information

A Novel WRKY Transcription Factor from Ipomoea trifida, ItfWRKY70, Confers Drought Tolerance in Sweet Potato.

Publications

A Novel WRKY Transcription Factor from <i>Ipomoea trifida</i>, ItfWRKY70, Confers Drought Tolerance in Sweet Potato.

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