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Co-Silencing of the Voltage-Gated Calcium Channel β Subunit and High-Voltage Activated α₁ Subunit by dsRNA Soaking Resulted in Enhanced Defects in Locomotion, Stylet Thrusting, Chemotaxis, Protein Secretion, and Reproduction in Ditylenchus destructor.

ABSTRACT: The voltage-gated calcium channel (VGCC) β subunit (Ca_vβ) protein is a kind of cytosolic auxiliary subunit that plays an important role in regulating the surface expression and gating characteristics of high-voltage-activated (HVA) calcium channels. Ditylenchus destructor is an important plant-parasitic nematode. In the present study, the putative Ca_vβ subunit gene of D. destructor, namely, DdCa_vβ, was subjected to molecular characterization. In situ hybridization assays showed that DdCa_vβ was expressed in all nematode tissues. Transcriptional analyses showed that DdCa_vβ was expressed during each developmental stage of D. destructor, and the highest expression level was recorded in the third-stage juveniles. The crucial role of DdCa_vβ was verified by dsRNA soaking-mediated RNA interference (RNAi). Silencing of DdCa_vβ or HVA Ca_vα₁ alone and co-silencing of the DdCa_vβ and HVA Ca_vα₁ genes resulted in defective locomotion, stylet thrusting, chemotaxis, protein secretion and reproduction in D. destructor. Co-silencing of the HVA Ca_vα₁ and Ca_vβ subunits showed stronger interference effects than single-gene silencing. This study provides insights for further study of VGCCs in plant-parasitic nematodes.

SUBMITTER: An M

PROVIDER: S-EPMC8776034 | biostudies-literature |

REPOSITORIES: biostudies-literature

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