Unknown

Dataset Information

0

Dracunculin Inhibits Adipogenesis in Human Bone Marrow-Derived Mesenchymal Stromal Cells by Activating AMPK and Wnt/β-Catenin Signaling.


ABSTRACT: Increased bone marrow adiposity is widely observed in patients with obesity and osteoporosis and reported to have deleterious effects on bone formation. Dracunculin (DCC) is a coumarin isolated from Artemisia spp. but, until now, has not been studied for its bioactive potential except antitrypanosomal activity. In this context, current study has reported the anti-adipogenic effect of DCC in human bone marrow-derived mesenchymal stromal cells (hBM-MSCs). DCC dose-dependently inhibited the lipid accumulation and expression of adipogenic transcription factors peroxisome proliferator-activated receptor γ (PPARγ) and CCAAT/enhancer binding protein α (C/EBPα) in hBM-MSCs induced to undergo adipogenesis. To elucidate its action mechanism, the effect of DCC on Wnt/β-catenin and AMPK pathways was examined. Results showed that DCC treatment activated Wnt/β-catenin signaling pathway via AMPK evidenced by increased levels of AMPK phosphorylation and Wnt10b expression after DCC treatment. In addition, DCC treated adipo-induced hBM-MSCs exhibited significantly increased nuclear levels of β-catenin compared with diminished nuclear PPARγ levels. In conclusion, DCC was shown to be able to hinder adipogenesis by activating the β-catenin via AMPK, providing potential utilization of DCC as a nutraceutical against bone marrow adiposity.

SUBMITTER: Karadeniz F 

PROVIDER: S-EPMC8776130 | biostudies-literature |

REPOSITORIES: biostudies-literature

Similar Datasets

| S-EPMC5854628 | biostudies-literature
| S-EPMC4406869 | biostudies-literature
| S-EPMC10599756 | biostudies-literature
| S-EPMC6234553 | biostudies-literature
| S-EPMC4900133 | biostudies-literature
| S-EPMC8110949 | biostudies-literature
| S-EPMC7847333 | biostudies-literature
| S-EPMC8183571 | biostudies-literature
| S-EPMC6400381 | biostudies-literature
| S-EPMC7648692 | biostudies-literature