Ontology highlight
ABSTRACT:
SUBMITTER: Rombout YB
PROVIDER: S-EPMC87791 | biostudies-literature | 2001 Feb
REPOSITORIES: biostudies-literature
Rombout Y B YB Bosch S S Van Der Giessen J W JW
Journal of clinical microbiology 20010201 2
A reverse line blot (RLB) assay was developed to identify different Trichinella genotypes. The RLB assay accomplishes detection and specific identification of the different Trichinella genotypes and relies on hybridization of the amplified 5S ribosomal DNA intergenic spacer regions to specific, membrane-bound oligonucleotide probes. After one single amplification, we were able to detect and genetically identify six sibling species, i.e., T. spiralis, T. britovi, T. nativa, T. murrelli, T. nelson ...[more]