Dendritic cell-mediated chronic low-grade inflammation is regulated by the RAGE-TLR4-PKCβ₁ signaling pathway in diabetic atherosclerosis.

ABSTRACT:

Background

The unique mechanism of diabetic atherosclerosis has been a central research focus. Previous literature has reported that the inflammatory response mediated by dendritic cells (DCs) plays a vital role in the progression of atherosclerosis. The objective of the study was to explore the role of DCs in diabetes mellitus complicated by atherosclerosis.

Methods

ApoE^-/- mice and bone marrow-derived DCs were used for in vivo and in vitro experiments, respectively. Masson's staining and Oil-red-O staining were performed for atherosclerotic lesion assessment. The content of macrophages and DCs in plaque was visualized by immunohistochemistry. The expression of CD83 and CD86 were detected by flow cytometry. The fluctuations in the RNA levels of cytokines, chemoki

SUBMITTER: Zhao L 

PROVIDER: S-EPMC8780245 | biostudies-literature | 2022 Jan

REPOSITORIES: biostudies-literature