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Assessment of human adipose‐derived stem cell on surface‐modified silicone implant to reduce capsular contracture formation

ABSTRACT: Abstract Medical devices made from poly(dimethylsiloxane) (PDMS)‐based silicone implants have been broadly used owing to their inert properties, biocompatibility, and low toxicity. However, long‐term implantation is usually associated with complications, such as capsular contracture due to excessive local inflammatory response, subsequently requiring implant removal. Therefore, modification of the silicone surface to reduce a risk of capsular contracture has attracted increasing attention. Human adipose‐derived stem cells (hASCs) are known to provide potentially therapeutic applications for tissue engineering, regenerative medicine, and reconstructive surgery. Herein, hASCs coating on a PDMS (hASC‐PDMS) or itaconic acid (IA)‐conjugated PDMS (hASC‐IA‐PDMS) surface is examined to determine its biocompatibility for reducing capsular contracture on the PDMS surface. In vitro cell cytotoxicity evaluation showed that hASCs on IA‐PDMS exhibit higher cell viability than hASCs on PDMS. A lower release of proinflammatory cytokines is observed in hASC‐PDMS and hASC‐IA‐PDMS compared to the cells on plate. Multiple factors, including in vivo mRNA expression levels of cytokines related to fibrosis; number of inflammatory cells; number of macrophages and myofibroblasts; capsule thickness; and collagen density following implantation in rats for 60 days, indicate that incorporated coating hASCs on PDMSs most effectively reduces capsular contracture. This study demonstrates the potential of hASCs coating for the modification of PDMS surfaces in enhancing surface biocompatibility for reducing capsular contracture of PDMS‐based medical devices.

SUBMITTER: Sutthiwanjampa C

PROVIDER: S-EPMC8780897 | biostudies-literature |

REPOSITORIES: biostudies-literature

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Poly Implant Prothese silicone breast implants: implant dynamics and capsular contracture.

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| S-EPMC6153863 | biostudies-other

Surface Topography, Microbial Adhesion, and Immune Responses in Silicone Mammary Implant-Associated Capsular Fibrosis.

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| S-EPMC10969816 | biostudies-literature

The low abundance plasma proteome reveals changes associ-ated with breast implant capsular contracture

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2024-10-17 | PXD044649 | Pride

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The Low-Abundance Plasma Proteome Reveals Differentially Abundant Proteins Associated with Breast Implant Capsular Contracture: A Pilot Study.

Project description:Capsular contracture (CC) is one of the most common postoperative complications associated with breast implant-associated infections. The mechanisms that lead to CC remain poorly understood. Plasma is an ideal biospecimen for early proteomics biomarker discovery. However, as high-abundance proteins mask signals from low-abundance proteins, identifying novel or specific proteins as biomarkers for a particular disease has been hampered. Here, we employed depletion of high-abundance plasma proteins followed by Tandem Mass Tag (TMT)-based quantitative proteomics to compare 10 healthy control patients against 10 breast implant CC patients. A total of 450 proteins were identified from these samples. Among them, 16 proteins were significantly differentially expressed in which 5 proteins were upregulated and 11 downregulated in breast implant CC patients compared to healthy controls. Gene Ontology enrichment analysis revealed that proteins related to cell, cellular processes and catalytic activity were highest in the cellular component, biological process, and molecular function categories, respectively. Further, pathway analysis revealed that inflammatory responses, focal adhesion, platelet activation, and complement and coagulation cascades were enriched pathways. The differentially abundant proteins from TMT-based quantitative proteomics have the potential to provide important information for future mechanistic studies and in the development of breast implant CC biomarkers.

| S-EPMC11348101 | biostudies-literature

Oral doxycycline prevents skin-associated adverse effects induced by injectable collagenase in a rodent model of capsular contracture around silicone implants.

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| S-EPMC9258873 | biostudies-literature

Adipose stem cells exhibit mechanical memory and reduce fibrotic contracture in a rat elbow injury model.

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| S-EPMC8745456 | biostudies-literature

Combinational therapy with antibiotics and antibiotic-loaded adipose-derived stem cells reduce abscess formation in implant-related infection in rats.

Project description:Implant-related infection is difficult to treat without extended antibiotic courses. However, the long-term use of antibiotics has led to the development of multidrug- and methicillin-resistant Staphylococcus aureus. Thus, alternatives to conventional antibiotic therapy are needed. Recently, mesenchymal stem cells have been shown to have antimicrobial properties. This study aimed to evaluate the antimicrobial activity and therapeutic effect of local treatment with antibiotic-loaded adipose-derived stem cells (ADSCs) plus an antibiotic in a rat implant-associated infection model. Liquid chromatography/tandem mass spectrometry revealed that ADSCs cultured in the presence of ciprofloxacin for 24 h showed time-dependent antibiotic loading. Next, we studied the therapeutic effects of ADSCs and ciprofloxacin alone or in combination in an implant-related infection rat model. The therapeutic effects of ADSCs plus antibiotics, antibiotics, and ADSCs were compared with no treatment as a control. Rats treated with ADSCs plus ciprofloxacin had the lowest modified osteomyelitis scores, abscess formation, and bacterial burden on the implant among all groups (P < 0.05). Thus, local treatment with ADSCs plus an antibiotic has an antimicrobial effect in implant-related infection and decrease abscess formation. Thus, our findings indicate that local administration of ADSCs with antibiotics represents a novel treatment strategy for implant-associated osteomyelitis.

| S-EPMC7341734 | biostudies-literature

Influence of Surface Modified Nanodiamonds on Dielectric and Mechanical Properties of Silicone Composites.

Project description:Detonation nanodiamonds, also known as ultradispersed diamonds, possess versatile chemically active surfaces, which can be adjusted to improve their interaction with elastomers. Such improvements can result in decreased dielectric and viscous losses of the composites without compromising other in-rubber properties, thus making the composites suitable for new demanding applications, such as energy harvesting. However, in most cases, surface modification of nanodiamonds requires the use of strong chemicals and high temperatures. The present study offers a less time-consuming functionalization method at 40 °C via reaction between the epoxy-rings of the modifier and carboxylic groups at the nanodiamond surface. This allows decorating the nanodiamond surface with chemical groups that are able to participate in the crosslinking reaction, thus creating strong interaction between filler and elastomer. Addition of 0.1 phr (parts per hundred rubber) of modified nanodiamonds into the silicone matrix results in about fivefold decreased electric losses at 1 Hz due to a reduced conductivity. Moreover, the mechanical hysteresis loss is reduced more than 50% and dynamic loss tangent at ambient temperature is lowered. Therefore, such materials are recommended for the dielectric energy harvesting application, and they are expected to increase its efficiency.

| S-EPMC6681107 | biostudies-literature

Subfascial Breast Augmentation: A Systematic Review and Meta-Analysis of Capsular Contracture.

Project description:BackgroundSubfascial breast augmentation is a technique originally developed to reduce the risks of capsular contracture while decreasing the postoperative pain associated with subpectoral augmentation. It was pioneered in Brazil by Dr. Graf and others, and recently this technique has gained interest in the aesthetic world.ObjectivesThe goal of this study was to provide a systematic analysis of subfascial breast augmentation to assess the combined reported rates of capsular contracture, animation deformity and complications.MethodsThe PubMed, Embase, and Web of Science databases were searched for the use of the subfascial plane for breast augmentation. We included studies that reported on capsular contracture and other outcomes following subfascial breast augmentation.ResultsThrough the initial search, 26 articles were identified. Of which, 22 were included in the final study. A total of 3743 patients were identified across these studies with a total number of 38 cases of capsular contracture representing a rate of 1.01% of capsular contracture. Several articles reported on demographics, perioperative and long-term complications, and outcomes with regards to the aesthetic outcome from the surgeon's perspective. Several infections were reported representing a rate of 0.1%. Animation deformity was not reported, although rippling was occasionally reported as was malrotation, axillary banding, sensory deficit, and asymmetry. Subfascial breast augmentation appears to have a low complication rate and an extremely low rate of capsular contracture at approximately 1%.ConclusionsSubfascial breast augmentation may provide the benefits of low rates of capsular contracture while avoiding the discomfort and future animation deformity of subpectoral augmentation.Level of evidence 4

| S-EPMC7671235 | biostudies-literature

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