Ontology highlight
ABSTRACT:
SUBMITTER: Khan MF
PROVIDER: S-EPMC8783101 | biostudies-literature | 2022 Mar
REPOSITORIES: biostudies-literature
Khan Mohd Faheem MF Murphy Cormac D CD
Biochemistry and biophysics reports 20220117
The microbial model of mammalian drug metabolism, <i>Cunninghamella elegans</i>, has three cytochrome P450 reductase genes in its genome: g1631 (CPR_A), g4301 (CPR_B), and g7609 (CPR_C). The nitroreductase activity of the encoded enzymes was investigated via expression of the genes in the yeast <i>Pichia pastoris</i> X33. Whole cell assays with the recombinant yeast demonstrated that the reductases converted the anticancer drug flutamide to the nitroreduced metabolite that was also produced from ...[more]