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Multi-CUT&Tag to simultaneously profile multiple chromatin factors.

ABSTRACT: Genome-wide chromatin mapping approaches typically focus on one protein at a time. We recently developed multi-CUT&Tag, which enables simultaneous mapping of multiple chromatin proteins in the same single cells or pools of cells. Using barcoded adapters loaded onto antibody-protein A-Tn5 transposase complexes, multi-CUT&Tag marks the locations of each chromatin protein and directly detects colocalization of different proteins in the same cell(s). Although slightly more laborious than CUT&Tag, multi-CUT&Tag provides a powerful option for generating multi-factor maps for epigenomic profiling. For complete details on the use and execution of this protocol, please refer to Gopalan et al. (2021).

SUBMITTER: Gopalan S

PROVIDER: S-EPMC8783141 | biostudies-literature | 2022 Mar

REPOSITORIES: biostudies-literature

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Multi-CUT&Tag to simultaneously profile multiple chromatin factors.

Gopalan Sneha S Fazzio Thomas G TG

STAR protocols 20220120 1

Genome-wide chromatin mapping approaches typically focus on one protein at a time. We recently developed multi-CUT&Tag, which enables simultaneous mapping of multiple chromatin proteins in the same single cells or pools of cells. Using barcoded adapters loaded onto antibody-protein A-Tn5 transposase complexes, multi-CUT&Tag marks the locations of each chromatin protein and directly detects colocalization of different proteins in the same cell(s). Although slightly more laborious than CUT&Tag, mu ...[more]

PMID: 35098158

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