Unknown

Dataset Information

0

Facile isolation of high-affinity nanobodies from synthetic libraries using CDR-swapping mutagenesis.


ABSTRACT: The generation of high-affinity nanobodies for diverse biomedical applications typically requires immunization or affinity maturation. Here, we report a simple protocol using complementarity-determining region (CDR)-swapping mutagenesis to isolate high-affinity nanobodies from common framework libraries. This approach involves shuffling the CDRs of low-affinity variants during the sorting of yeast-displayed libraries to directly isolate high-affinity nanobodies without the need for lead isolation and optimization. We expect this approach, which we demonstrate for SARS-CoV-2 neutralizing nanobodies, will simplify the generation of high-affinity nanobodies. For complete details on the use and execution of this profile, please refer to Zupancic et al. (2021).

SUBMITTER: Zupancic JM 

PROVIDER: S-EPMC8783142 | biostudies-literature |

REPOSITORIES: biostudies-literature

Similar Datasets

| S-EPMC8223476 | biostudies-literature
| S-EPMC4404190 | biostudies-literature
| S-EPMC5591402 | biostudies-literature
| S-EPMC6497274 | biostudies-literature
| S-EPMC438952 | biostudies-literature
| S-EPMC5977401 | biostudies-literature
2023-06-21 | GSE202972 | GEO
| S-EPMC6629726 | biostudies-literature
| S-EPMC7443875 | biostudies-literature
| S-EPMC5818986 | biostudies-literature