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Two photon imaging of calcium responses in murine Purkinje neurons.

ABSTRACT: Purkinje neurons (PNs) are an important component of the motor learning and coordination circuit and are affected in spino-cerebellar ataxias. Maintaining healthy PNs in cerebellar slices and recording their Ca²⁺ transients can be challenging. Here, we describe a protocol for measuring Ca²⁺ transients in PNs from adult mice, including problem-solving tips. This protocol can be used to measure neuronal excitability and agonist-mediated Ca²⁺ signaling in cerebellar slices expressing a genetic Ca²⁺ reporter in all PNs, thus improving yield of data. For complete details on the use and execution of this profile, please refer to Dhanya and Hasan (2021).

SUBMITTER: Dhanya SK

PROVIDER: S-EPMC8783150 | biostudies-literature | 2022 Mar

REPOSITORIES: biostudies-literature

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Two photon imaging of calcium responses in murine Purkinje neurons.

Dhanya Sreeja Kumari SK Hasan Gaiti G

STAR protocols 20220120 1

Purkinje neurons (PNs) are an important component of the motor learning and coordination circuit and are affected in spino-cerebellar ataxias. Maintaining healthy PNs in cerebellar slices and recording their Ca2+ transients can be challenging. Here, we describe a protocol for measuring Ca2+ transients in PNs from adult mice, including problem-solving tips. This protocol can be used to measure neuronal excitability and agonist-mediated Ca2+ signaling in cerebellar ...[more]

PMID: 35098161

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