Statistics or biology: the zero-inflation controversy about scRNA-seq data.
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ABSTRACT:
SUBMITTER: Jiang R
PROVIDER: S-EPMC8783472 | biostudies-literature | 2022 Jan
REPOSITORIES: biostudies-literature
Publications
Statistics or biology: the zero-inflation controversy about scRNA-seq data.
Jiang Ruochen R Sun Tianyi T Song Dongyuan D Li Jingyi Jessica JJ
Genome biology 20220121 1
Researchers view vast zeros in single-cell RNA-seq data differently: some regard zeros as biological signals representing no or low gene expression, while others regard zeros as missing data to be corrected. To help address the controversy, here we discuss the sources of biological and non-biological zeros; introduce five mechanisms of adding non-biological zeros in computational benchmarking; evaluate the impacts of non-biological zeros on data analysis; benchmark three input data types: observ ...[more]
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