Temporal Modulation of Differential Alternative Splicing in HaCaT Human Keratinocyte Cell Line Chronically Exposed to Arsenic for up to 28 Wk.

ABSTRACT:

Background

Chronic arsenic exposure via drinking water is associated with an increased risk of developing cancer and noncancer chronic diseases. Pre-mRNAs are often subject to alternative splicing, generating mRNA isoforms encoding functionally distinct protein isoforms. The resulting imbalance in isoform species can result in pathogenic changes in critical signaling pathways. Alternative splicing as a mechanism of arsenic-induced toxicity and carcinogenicity is understudied.

Objective

This study aimed to accurately profile differential alternative splicing events in human keratinocytes induced by chronic arsenic exposure that might play a role in carcinogenesis.

Methods

Independent quadruplicate cultures of immortalized human keratinocytes (HaCaT) were maintained con

SUBMITTER: Ferragut Cardoso AP 

PROVIDER: S-EPMC8785870 | biostudies-literature | 2022 Jan

REPOSITORIES: biostudies-literature