ABSTRACT: The complete cp genome of Prunus tangutica is 158,131 bp in length, exhibits a typical quadripartite structural organization, consisting of a large single copy (LSC) region of 86,266 bp, two inverted repeats (IR) regions of 26,389 bp, and a small single copy (SSC) region of 19,087 bp. The cp genome contains 131 complete genes, including 86 protein-coding genes (86 PCGs), 8 ribosomal RNA genes (8 rRNAs), and 37 tRNA genes (37 tRNAs). Most genes occur in a single copy, while 19 genes occur in double, including 4 rRNAs (4.5S, 5S, 16S, and 23S rRNA), 7 tRNAs (trnA-UGC, trnI-GAU, trnL-CAA, trnI-CAU, trnN-GUU, trnR-ACG, and trnV-GAC), and 5 PCGs (rps7, ndhB, ycf2, rpl2 and rpl23). The overall GC content of cp DNA is 36.7%, the corresponding values of the LSC, SSC, and IR regions are 34.6%, 30.1%, and 42.6%, respectively. Further, the phylogenetic analysis suggested that the P. tangutica was closely related to Prunus tenella. The results of P. tangutica will lay a foundation for further research.