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Nonalternating purine pyrimidine sequences can form stable left-handed DNA duplex by strong topological constraint.

ABSTRACT: In vivo, left-handed DNA duplex (usually refers to Z-DNA) is mainly formed in the region of DNA with alternating purine pyrimidine (APP) sequence and plays significant biological roles. It is well known that d(CG)n sequence can form Z-DNA most easily under negative supercoil conditions, but its essence has not been well clarified. The study on sequence dependence of Z-DNA stability is very difficult without modification or inducers. Here, by the strong topological constraint caused by hybridization of two complementary short circular ssDNAs, left-handed duplex part was generated for various sequences, and their characteristics were investigated by using gel-shift after binding to specific proteins, CD and Tm analysis, and restriction enzyme cleavage. Under the strong topological constraint, non-APP sequences can also form left-handed DNA duplex as stable as that of APP sequences. As compared with non-APP sequences, the thermal stability difference for APP sequences between Z-form and B-form is smaller, which may be the reason that Z-DNA forms preferentially for APP ones. This result can help us to understand why nature selected APP sequences to regulate gene expression by transient Z-DNA formation, as well as why polymer with chirality can usually form both duplexes with left- or right-handed helix.

SUBMITTER: Li L

PROVIDER: S-EPMC8789069 | biostudies-literature |

REPOSITORIES: biostudies-literature

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Project description:The purpose of this study was to examine performance advantages associated with batting stance, in the form of left- vs. right-handed dominant stance, and orthodox vs. reverse stance, of talented junior cricket batters within age-restricted competitions. Data were sourced from the national male younger age competition (YAC; Under-17; n = 237) and older age competition (OAC; Under-19; n = 302), as well as female YAC (Under-15; n = 234) and OAC (Under-18; n = 260) over a 4-year period. Left-hand dominant (LHD) batters were consistently overrepresented in the male YAC (Right: 69.2%; Left: 30.8%) and OAC (Right: 68.2%; Left: 31.8%) compared with the expected general population distribution. Male LHD batters exhibited a significantly (p < 0.05) higher batting aggregate (YAC: 116.82 ± 84.75 vs. 137.84 ± 89.74; OAC: 117.07 ± 89.00 vs. 146.28 ± 95.99), scored more runs (YAC: 19.65 ± 12.32 vs. 23.96 ± 14.71; OAC: 19.27 ± 12.61 vs. 23.98 ± 14.15), spent more time batting (YAC: 45.33 ± 25.89 min vs. 54.59 ± 28.62 min; OAC: 39.80 ± 21.79 min vs. 49.33 ± 27.41 min), and scored more boundary-4s per game (YAC: 1.83 ± 1.40 vs. 2.44 ± 1.87; OAC: 1.76 ± 1.32 vs. 2.19 ± 1.83), across both YAC and OAC groups with small effect sizes. No overrepresentation was present for either female group (YAC, Right: 88.5%/Left: 11.5%; OAC, Right: 90.0%/Left: 10.0%). Female LHD batters exhibited significantly higher batting aggregate (68.97 ± 53.17 vs. 102.96 ± 73.48), batting average (13.24 ± 10.88 vs. 17.75 ± 12.28), and spent more time batting per game (25.52 ± 15.08 vs. 37.75 ± 26.76 min), but only at the OAC level with small-moderate effects sizes. Finally, there were few performance advantages identified to batting with a reverse stance, with further work needed to clarify any potential biomechanical benefits. Team selection practices may exploit the left-handed advantage by over-selecting talented left-handed junior cricketers. Practical implications for coaches include creating practice environments that negate the negative frequency-dependent selection, such as providing more practice opportunities for their players against left-handed opponents.

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Nonalternating purine pyrimidine sequences can form stable left-handed DNA duplex by strong topological constraint.

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