Juxtacellular opto-tagging of hippocampal CA1 neurons in freely moving mice.

ABSTRACT: Neural circuits are made of a vast diversity of neuronal cell types. While immense progress has been made in classifying neurons based on morphological, molecular, and functional properties, understanding how this heterogeneity contributes to brain function during natural behavior has remained largely unresolved. In the present study, we combined the juxtacellular recording and labeling technique with optogenetics in freely moving mice. This allowed us to selectively target molecularly defined cell classes for in vivo single-cell recordings and morphological analysis. We validated this strategy in the CA1 region of the mouse hippocampus by restricting Channelrhodopsin expression to Calbindin-positive neurons. Directly versus indirectly light-activated neurons could be readily distinguished

SUBMITTER: Ding L 

PROVIDER: S-EPMC8791633 | biostudies-literature | 2022 Jan

REPOSITORIES: biostudies-literature