Dataset Information

Human blastoids model blastocyst development and implantation.

ABSTRACT: One week after fertilization, human embryos implant into the uterus. This event requires the embryo to form a blastocyst consisting of a sphere encircling a cavity lodging the embryo proper. Stem cells can form a blastocyst model that we called a blastoid¹. Here we show that naive human pluripotent stem cells cultured in PXGL medium² and triply inhibited for the Hippo, TGF-β and ERK pathways efficiently (with more than 70% efficiency) form blastoids generating blastocyst-stage analogues of the three founding lineages (more than 97% trophectoderm, epiblast and primitive endoderm) according to the sequence and timing of blastocyst development. Blastoids spontaneously form the first axis, and we observe that the epiblast induces the local maturation of the polar trophectoderm, thereby endowing blastoids with the capacity to directionally attach to hormonally stimulated endometrial cells, as during implantation. Thus, we propose that such a human blastoid is a faithful, scalable and ethical model for investigating human implantation and development^3,4.

SUBMITTER: Kagawa H

PROVIDER: S-EPMC8791832 | biostudies-literature | 2022 Jan

REPOSITORIES: biostudies-literature

ACCESS DATA

Publications

Human blastoids model blastocyst development and implantation.

Kagawa Harunobu H Javali Alok A Khoei Heidar Heidari HH Sommer Theresa Maria TM Sestini Giovanni G Novatchkova Maria M Scholte Op Reimer Yvonne Y Castel Gaël G Bruneau Alexandre A Maenhoudt Nina N Lammers Jenna J Loubersac Sophie S Freour Thomas T Vankelecom Hugo H David Laurent L Rivron Nicolas N

Nature 20211202 7894

One week after fertilization, human embryos implant into the uterus. This event requires the embryo to form a blastocyst consisting of a sphere encircling a cavity lodging the embryo proper. Stem cells can form a blastocyst model that we called a blastoid<sup>1</sup>. Here we show that naive human pluripotent stem cells cultured in PXGL medium<sup>2</sup> and triply inhibited for the Hippo, TGF-β and ERK pathways efficiently (with more than 70% efficiency) form blastoids generating blastocyst-st ...[more]

PMID: 34856602

Similar Datasets

Project description:Study questionIs relative mitochondrial DNA (mtDNA) content in cumulus cells (CCs) related to embryo developmental competence in humans and/or the bovine model?Summary answermtDNA content in CCs provides a poor predictive value of oocyte developmental potential, both in vitro and following embryo transfer.What is known alreadyCCs are closely connected to the oocyte through transzonal projections, serving essential metabolic functions during folliculogenesis. These oocyte-supporting cells are removed and discarded prior to ICSI, thereby providing interesting biological material on which to perform molecular analyses designed to identify markers that predict oocyte developmental competence. Previous studies have positively associated oocyte mtDNA content with developmental potential in animal models and women. However, it remains debatable whether mtDNA content in CCs could be used as a proxy to infer oocyte developmental potential.Study design size durationmtDNA content was analyzed in CCs obtained from 109 human oocytes unable to develop to blastocyst, able to develop to blastocyst but failing to establish pregnancy or able to develop to blastocyst and to establish pregnancy. mtDNA analysis was also performed on bovine cumulus samples collected from 120 oocytes unable to cleave, oocytes developing into cleaved embryos but arresting development prior to the blastocyst stage or oocytes developing to blastocysts.Participants/materials setting methodsHuman CCs samples were obtained from women undergoing IVF. Only unfrozen oocytes and embryos not submitted to preimplantation genetic testing were included in the analysis. Bovine samples were obtained from slaughtered cattle and individually matured, fertilized and cultured in vitro. Relative mtDNA was assessed by quantitative PCR analysis.Main results and the role of chancemtDNA content in human and bovine CCs did not differ according to the developmental potential of their enclosed oocyte. Moreover, mtDNA content in bovine oocytes did not correlate with that of their corresponding CCs.Large scale dataN/A.Limitations reasons for cautionThe lack of correlation found between mtDNA content in human CCs and oocytes was also assessed in bovine samples. Although bovine folliculogenesis, mono-ovulatory ovulation and early embryo development exhibit considerable similarities with that of humans, they may not be fully comparable.Wider implications of the findingsThe use of molecular markers for oocyte developmental potential in CCs could be used to enhance success rates following single embryo transfer. However, our data indicate that mtDNA in CCs is not a good proxy for oocyte quality.Study funding/competing interestsThis research was supported by the Industrial Doctorate Project IND2017/BIO-7748 funded by the Madrid Region Government. The authors declare no competing interests.

Dataset Information

Human blastoids model blastocyst development and implantation.

Publications

Human blastoids model blastocyst development and implantation.

Similar Datasets

OmicsDI is part of the ELIXIR infrastructure

Tweets