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Mechanosensitive channel Piezo1 induces cell apoptosis in pancreatic cancer by ultrasound with microbubbles

ABSTRACT: Summary Ultrasound (US), as a safe and non-invasive tool, has drawn researchers' attention to treat pancreatic ductal adenocarcinoma (PDAC). Piezo1, a mechanosensitive channel, can be activated by various mechanical stimuli. In this study, we tested the expression of Piezo1 in PDAC cell lines and tissues, and cell apoptosis in vitro and in vivo with siRNA, a lentivirus system, and a subcutaneous xenograft tumor-bearing model under the condition of US with microbubbles (MBs). We found that Piezo1 was highly expressed in PDAC cells; it was activated by US with MBs and was closely related to the apoptosis of PDAC cell lines and tumors. This study highlighted the idea of utilizing the high expression of Piezo1 in PDAC and US with MBs to provide a non-invasive strategy for the treatment of PDAC from the aspect of mechanotransduction. Graphical abstract Highlights • Mechanosensitive channel Piezo1 is highly expressed in pancreatic cancer cells• Ultrasound with microbubbles induces apoptosis of pancreatic cancer cells• Piezo1 is activated by ultrasound with microbubbles and mediates calcium influx Molecular biology; Cell biology; Cancer

SUBMITTER: Song Y

PROVIDER: S-EPMC8792083 | biostudies-literature |

REPOSITORIES: biostudies-literature

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