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Transcriptome and Proteomics Analysis of Wheat Seedling Roots Reveals That Increasing NH₄ ⁺/NO₃ ^- Ratio Induced Root Lignification and Reduced Nitrogen Utilization.

ABSTRACT: Wheat growth and nitrogen (N) uptake gradually decrease in response to high NH₄ ⁺/NO₃ ^- ratio. However, the mechanisms underlying the response of wheat seedling roots to changes in NH₄ ⁺/NO₃ ^- ratio remain unclear. In this study, we investigated wheat growth, transcriptome, and proteome profiles of roots in response to increasing NH₄ ⁺/NO₃ ^- ratios (N _a : 100/0; N _r1: 75/25, N _r2: 50/50, N _r3: 25/75, and N _n : 0/100). High NH₄ ⁺/NO₃ ^- ratio significantly reduced leaf relative chlorophyll content, Fv/Fm, and ΦII values. Both total root length and specific root length decreased with increasing NH₄ ⁺/NO₃ ^- ratios. Moreover, the rise in NH₄ ⁺/NO₃ ^- ratio significantly promoted O₂ ^- production. Furthermore, transcriptome sequencing and tandem mass tag-based quantitative proteome analyses identified 14,376 differentially expressed genes (DEGs) and 1,819 differentially expressed proteins (DEPs). The Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment analysis indicated that glutathione metabolism and phenylpropanoid biosynthesis were the main two shared enriched pathways across ratio comparisons. Upregulated DEGs and DEPs involving glutathione S-transferases may contribute to the prevention of oxidative stress. An increment in the NH₄ ⁺/NO₃ ^- ratio induced the expression of genes and proteins involved in lignin biosynthesis, which increased root lignin content. Additionally, phylogenetic tree analysis showed that both A0A3B6NPP6 and A0A3B6LM09 belong to the cinnamyl-alcohol dehydrogenase subfamily. Fifteen downregulated DEGs were identified as high-affinity nitrate transporters or nitrate transporters. Upregulated TraesCS3D02G344800 and TraesCS3A02G350800 were involved in ammonium transport. Downregulated A0A3B6Q9B3 is involved in nitrate transport, whereas A0A3B6PQS3 is a ferredoxin-nitrite reductase. This may explain why an increase in the NH₄ ⁺/NO₃ ^- ratio significantly reduced root NO₃ ^--N content but increased NH₄ ⁺-N content. Overall, these results demonstrated that increasing the NH₄ ⁺/NO₃ ^- ratio at the seedling stage induced the accumulation of reactive oxygen species, which in turn enhanced root glutathione metabolism and lignification, thereby resulting in increased root oxidative tolerance at the cost of reducing nitrate transport and utilization, which reduced leaf photosynthetic capacity and, ultimately, plant biomass accumulation.

SUBMITTER: Yang D

PROVIDER: S-EPMC8792948 | biostudies-literature |

REPOSITORIES: biostudies-literature

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Project description:BackgroundIn northwest of China, mini Chinese cabbage (Brassica pekinensis) is highly valued by consumers, and is widely cultivated during winter in solar-greenhouses where low light (LL) fluence (between 85 and 150 μmol m-2 s-1 in day) is a major abiotic stress factor limiting plant growth and crop productivity. The mechanisms with which various NH4+: NO3- ratios affected growth and photosynthesis of mini Chinese cabbage under normal (200 μmol m-2 s-1) and low (100 μmol m-2 s-1) light conditions was investigated. The four solutions with different ratios of NH4+: NO3- applied were 0:100, 10:90, 15:85 and 25:75 with the set up in a glasshouse in hydroponic culture. The most appropriate NH4+: NO3- ratio that improved the tolerance of mini Chinese cabbage seedlings to LL was found in our current study.ResultsUnder low light, the application of NH4+: NO3- (10:90) significantly stimulated growth compared to only NO3- by increasing leaf area, canopy spread, biomass accumulation, and net photosynthetic rate. The increase in net photosynthetic rate was associated with an increase in: 1) maximum and effective quantum yield of PSII; 2) activities of Calvin cycle enzymes; and 3) levels of mRNA relative expression of several genes involved in Calvin cycle. In addition, glucose, fructose, sucrose, starch and total carbohydrate, which are the products of CO2 assimilation, accumulated most in the cabbage leaves that were supplied with NH4+: NO3- (10:90) under LL condition. Low light reduced the carbohydrate: nitrogen (C: N) ratio while the application of NH4+: NO3- (10:90) alleviated the negative effect of LL on C: N ratio mainly by increasing total carbohydrate contents.ConclusionsThe application of NH4+:NO3- (10:90) increased rbcL, rbcS, FBA, FBPase and TK expression and/or activities, enhanced photosynthesis, carbohydrate accumulation and improved the tolerance of mini Chinese cabbage seedlings to LL. The results of this study would provide theoretical basis and technical guidance for mini Chinese cabbage production. In practical production, the ratio of NH4+:NO3- should be adjusted with respect to light fluence for successful growing of mini Chinese cabbage.

Project description:Compared with sole nitrogen (N), the nutrition mixture of ammonium (NH4 +) and nitrate (NO3 -) is known to better improve crop yield and quality. However, the mechanism underlying this improvement remains unclear. In the present study, we analyzed the changes in nutrient solution composition, content of different N forms in plant tissues and exudates, and expression of plasma membrane (PM) H+-ATPase genes (HAs) under different NH4 +/NO3 - ratios (0/100, 10/90, 25/75, 50/50 as control, T1, T2, and T3) in flowering Chinese cabbage. We observed that compared with the control, T1 and T2 increased the economical yield of flowering Chinese cabbage by 1.26- and 1.54-fold, respectively, whereas T3 significantly reduced plant yield. Compared with the control, T1-T3 significantly reduced the NO3 - content and increased the NH4 +, amino acid, and soluble protein contents of flowering Chinese cabbage to varying extents. T2 significantly increased the N use efficiency (NUE), whereas T3 significantly decreased it to only being 70.25% of that of the control. Owing to the difference in N absorption and utilization among seedlings, the pH value of the nutrient solution differed under different NH4 +/NO3 - ratios. At harvest, the pH value of T2 was 5.8; in the control and T1, it was approximately 8.0, and in T3 it was only 3.6. We speculated that appropriate NH4 +/NO3 - ratios may improve N absorption and assimilation and thus promote the growth of flowering Chinese cabbage, owing to the suitable pH value. On the contrary, addition of excessive NH4 + may induce rhizosphere acidification and ammonia toxicity, causing plant growth inhibition. We further analyzed the transcription of PM H+-ATPase genes (HAs). HA1 and HA7 transcription in roots was significantly down-regulated by the addition of the mixture of NH4 + and NO3 -, whereas the transcription of HA2, HA9 in roots and HA7, HA8, and HA10 in leaves was sharply up-regulated by the addition of the mixture; the transcription of HA3 was mainly enhanced by the highest ratio of NH4 +/NO3 -. Our results provide valuable information about the effects of treatments with different NH4 +/NO3 - ratios on plant growth and N uptake and utilization.

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Transcriptome and Proteomics Analysis of Wheat Seedling Roots Reveals That Increasing NH₄ ⁺/NO₃ ^- Ratio Induced Root Lignification and Reduced Nitrogen Utilization.

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Transcriptome and Proteomics Analysis of Wheat Seedling Roots Reveals That Increasing NH4 +/NO3 - Ratio Induced Root Lignification and Reduced Nitrogen Utilization.

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OmicsDI is part of the ELIXIR infrastructure

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Transcriptome and Proteomics Analysis of Wheat Seedling Roots Reveals That Increasing NH₄ ⁺/NO₃ ^- Ratio Induced Root Lignification and Reduced Nitrogen Utilization.