Core clock regulators in dexamethasone-treated HEK 293T cells at 4 h intervals.
Ontology highlight
ABSTRACT: Objective
The study of the circadian clock and its mechanisms is easily facilitated through clock resetting in cell culture. Among the various established synchronizers of the circadian clock in cell culture (temperature, serum shock, glucocorticoids), the artificial glucocorticoid Dexamethasone (DEX) is the most widely used. DEX treatment as a protocol to reset the circadian clock in culture gives simple readout with minimal laboratory requirements. Even though there are many studies regarding clock resetting in culture using DEX, reference points or expression patterns of core clock genes and their protein products are scarce and sometimes contradict other works with similar methodology. We synchronise a cell line of human origin with DEX to be used for studies on circadian rhythms.Results
We treat HEK 293T cells with DEX and describe the patterns of mRNA and proteins of core clock regulators, while making a clear point on how CLOCK is less than an ideal molecule to help monitor rhythms in this cell line.
SUBMITTER: Beta RAA
PROVIDER: S-EPMC8796574 | biostudies-literature | 2022 Jan
REPOSITORIES: biostudies-literature
Publications
Core clock regulators in dexamethasone-treated HEK 293T cells at 4 h intervals.
Beta Rafailia A A RAA Arsenopoulou Zoi V ZV Kanoura Amalia A Dalkidis Dimitrios D Avraamidou Rafaela R Balatsos Nikolaos A A NAA
BMC research notes 20220128 1
<h4>Objective</h4>The study of the circadian clock and its mechanisms is easily facilitated through clock resetting in cell culture. Among the various established synchronizers of the circadian clock in cell culture (temperature, serum shock, glucocorticoids), the artificial glucocorticoid Dexamethasone (DEX) is the most widely used. DEX treatment as a protocol to reset the circadian clock in culture gives simple readout with minimal laboratory requirements. Even though there are many studies re ...[more]
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