Ontology highlight
ABSTRACT:
SUBMITTER: Kibirige CN
PROVIDER: S-EPMC8799642 | biostudies-literature | 2022 Jan
REPOSITORIES: biostudies-literature
Kibirige C N CN Manak M M King D D Abel B B Hack H H Wooding D D Liu Y Y Fernandez N N Dalel J J Kaye Steve S Imami N N Jagodzinski L L Gilmour J J
Scientific reports 20220128 1
An LTR-based quantitative PCR (qPCR) assay was modified and optimized for the quantification of total HIV-1 nucleic acids in plasma and PBMC. TaqMan qPCR primers and probes were designed against the NCBI/LANL HIV-1 compendium database by analyzing sequences used in assays for sensitive cross-clade detection of HIV-1 as reported in the literature and elucidating regions of improved cross-subtype specificity. Inosine and mixed nucleotide bases were included at polymorphic sites. Real-time RT-qPCR ...[more]