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Molecular Prosthetics for Long-Term Functional Imaging with Fluorescent Reporters.

ABSTRACT: Voltage-sensitive fluorescent reporters can reveal fast changes in the membrane potential in neurons and cardiomyocytes. However, in many cases, illumination in the presence of the fluorescent reporters results in disruptions to the action potential shape that limits the length of recording sessions. We show here that a molecular prosthetic approach, previously limited to fluorophores, rather than indicators, can be used to substantially prolong imaging in neurons and cardiomyocytes.

SUBMITTER: Grenier V

PROVIDER: S-EPMC8802189 | biostudies-literature | 2022 Jan

REPOSITORIES: biostudies-literature

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Molecular Prosthetics for Long-Term Functional Imaging with Fluorescent Reporters.

Grenier Vincent V Martinez Kayli N KN Benlian Brittany R BR García-Almedina Derek M DM Raliski Benjamin K BK Boggess Steven C SC Maza Johnathan C JC Yang Samantha J SJ Gest Anneliese M M AMM Miller Evan W EW

ACS central science 20220113 1

Voltage-sensitive fluorescent reporters can reveal fast changes in the membrane potential in neurons and cardiomyocytes. However, in many cases, illumination in the presence of the fluorescent reporters results in disruptions to the action potential shape that limits the length of recording sessions. We show here that a molecular prosthetic approach, previously limited to fluorophores, rather than indicators, can be used to substantially prolong imaging in neurons and cardiomyocytes. ...[more]

PMID: 35111902

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