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FIONA1 is an RNA N⁶-methyladenosine methyltransferase affecting Arabidopsis photomorphogenesis and flowering.

ABSTRACT:

Background

N⁶-methyladenosine (m⁶A) mRNA modification is essential for mammalian and plant viability. The U6 m⁶A methyltransferases in other species regulate S-adenosylmethionine (SAM) homeostasis through installing m⁶A in pre-mRNAs of SAM synthetases. However, U6 m⁶A methyltransferase has not been characterized in Arabidopsis and little is known about its role in regulating photomorphogenesis and flowering.

Results

Here we characterize that FIONA1 is an Arabidopsis U6 m⁶A methyltransferase that installs m⁶A in U6 snRNA and a small subset of poly(A)⁺ RNA. Disruption of FIONA1 leads to phytochrome signaling-dependent hypocotyl elongation and photoperiod-independent early flowering. Distinct from mammalian METTL16 and worm METT-10, FIONA1 neither installs m⁶A in the mRNAs of Arabidopsis SAM synthetases nor affects their transcript expression levels under normal or high SAM conditions. We confirm that FIONA1 can methylate plant mRNA m⁶A motifs in vitro and in vivo. We further show that FIONA1 installs m⁶A in several phenotypic related transcripts, thereby affecting downstream mRNA stability and regulating phytochrome signaling and floral transition.

Conclusion

FIONA1 is functional as a U6 m⁶A methyltransferase in Arabidopsis, distinct from mammalian METTL16 and worm METT-10. Our results demonstrate that FIONA1-mediated m⁶A post-transcriptional regulation is an autonomous regulator for flowering and phytochrome signaling-dependent photomorphogenesis.

SUBMITTER: Wang C

PROVIDER: S-EPMC8802475 | biostudies-literature |

REPOSITORIES: biostudies-literature

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