Dataset Information

Molecular characterization and the first full sequencing genome of chicken infectious anemia virus (CIAV) in Iran.

ABSTRACT:

Background

The chicken infectious anemia virus (CIAV) is an important pathogen that causes severe immunosuppression in young chickens.

Aims

The study aims to characterize the genotype and full-length sequencing of CIAV strains in Iran.

Methods

First, the collected thymus samples were investigated by conventional PCR for CIAV detection. Second, one of the CIAV positive samples (UT-Zahraee) was chosen for full genome sequencing.

Results

Throughout 2017, we detected 13 CIAVs isolated from 40 broiler flocks of different provinces of Iran. A comparison of the complete sequences of the genome and homologies of the nucleotides revealed that UT-Zahraee had a high similarity with American and Egyptian CIAV isolates. Moreover, VP1 sequence analysis showed that UT-Zahraee shared high homology with previously reported Iranian CIAV strains, Chinese, and Egyptian isolates.

Conclusion

This study is the first report of full genome sequencing of CIAV strain from Iran. It will be beneficial to understand better the epidemiology and molecular characteristics of CIAV circulating in Iran.

SUBMITTER: Hosseini H

PROVIDER: S-EPMC8806177 | biostudies-literature | 2021

REPOSITORIES: biostudies-literature

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Publications

Molecular characterization and the first full sequencing genome of chicken infectious anemia virus (CIAV) in Iran.

Hosseini H H Majidi S S Ziafati Kafi Z Z Esmaeelzadeh Dizaji R R Ghalyanchilangeroudi A A

Iranian journal of veterinary research 20210101 4

<h4>Background</h4>The chicken infectious anemia virus (CIAV) is an important pathogen that causes severe immunosuppression in young chickens.<h4>Aims</h4>The study aims to characterize the genotype and full-length sequencing of CIAV strains in Iran.<h4>Methods</h4>First, the collected thymus samples were investigated by conventional PCR for CIAV detection. Second, one of the CIAV positive samples (UT-Zahraee) was chosen for full genome sequencing.<h4>Results</h4>Throughout 2017, we detected 13 ...[more]

PMID: 35126541

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Project description:AimThe present study was aimed to determine the prevalence of infectious bronchitis virus (IBV) as well as virus isolation, identification, and molecular characterization of various strains circulating in Bangladesh.Materials and methodsA total of 371 swabs and organ samples were collected from four types of chicken including layer, Sonali (local), broiler, and broiler breeder under eight districts (Rangpur, Bogura, Tangail, Dhaka, Gazipur, Mymensingh, Jamalpur, and Cumilla) during 2014-2016 in Bangladesh.ResultsOut of 371 samples, 65 samples were positive in reverse transcriptase polymerase chain reaction (RT-PCR) for molecular identification of IBV. The overall prevalence was 17.52% recorded and among the selected types of chicken, the highest prevalence of IBV was found in layer that was 42.22% followed by 17.24% in Sonali, 14.93% in broiler breeder, and lowest prevalence was 11.94% in broiler chicken, respectively. Moreover, the prevalence of IBV was recorded highest in aged chicken at 41-60 weeks, which was 54.55% in layer, 27.27% in Sonali, and, afterward, 14.68% was found in broiler breeder, respectively. Frequency of IBV more frequently in winter (22.67%) followed by rainy (15.87%) and summer season (11.58%). The highest prevalence of IBV was found Tangail district (41.67%) followed by Mymensingh (24.42%), Gazipur (19.32%), Dhaka (15.38%), Jamalpur (16.67%), Bogura (13.68%), Cumilla (5.88%), and Rangpur (9.26%), respectively. Samples that were found high positive in IBV RT-PCR (Ct value below 30) were subjected to inoculation into chicken egg embryo to observe characteristic changes in chicken embryo. Swabs and organ samples were processed and passaged in 9-day-old embryonated chicken eggs through allantoic cavity route. IBV virus suspected samples inoculated into chicken egg embryos after 3-5 passages showed dwarfing and curling of the embryos which are characteristic lesions of IBV. Allantoic fluid was collected from all inoculated eggs and performed partial sequencing of S1 gene for three isolates. After sequencing, the phylogenetic tree was constructed from the nucleotide sequences of IBV isolates. Two of the isolates are 4/91 IBV and another one matched with QX-like IBV.ConclusionThe results revealed that the three isolates from different places in Bangladesh were identified for the 1st time as which will help for IBV control strategy.

Dataset Information

Molecular characterization and the first full sequencing genome of chicken infectious anemia virus (CIAV) in Iran.

Background

Aims

Methods

Results

Conclusion

Publications

Molecular characterization and the first full sequencing genome of chicken infectious anemia virus (CIAV) in Iran.

Similar Datasets

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