Ontology highlight
ABSTRACT:
SUBMITTER: Jauregui LH
PROVIDER: S-EPMC88090 | biostudies-literature | 2001 Jun
REPOSITORIES: biostudies-literature
Jauregui L H LH Higgins J J Zarlenga D D Dubey J P JP Lunney J K JK
Journal of clinical microbiology 20010601 6
A highly sensitive and specific method has been developed to reproducibly detect and quantitate Toxoplasma gondii burden in animal tissue samples using T. gondii ITS1-derived primers and a fluorogenic probe via real-time PCR. Assay specificity was confirmed against a panel of DNA samples from T. gondii and other common protozoa as well as host animal tissue. This Toxo TaqMan assay was able to detect as little as 0.1 pg of T. gondii genomic DNA, which is equivalent to 1 T. gondii bradyzoite, and ...[more]